Phosphate Assay Kit

100 assays (manual) / 400 assays (auto-analyser)

Item No.  K-PHOS

  • Chemically defined detection method
  • All reagents stable for > 2 years after preparation
  • Rapid reaction (~ 20 min)
  • Mega-Calc™ software tool is available from our website for hassle-free raw data processing
  • Standard included
  • Suitable for manual and auto-analyser formats
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Expect availability in 2-4 weeks
Minimum quantity required: 1
This item must be ordered in multiples of one case. Quantity per case: 1

The Phosphate Assay Kit is a rapid, simple, reliable and accurate method for the specific measurement and analysis of phosphate in water, foodstuffs, beverages and other materials. The phosphate detection reaction employs the defined chemical substrate 2-amino-6-mercapto-7-methylpurine ribonucleoside (MESG). In the presence of phosphate, the substrate is converted enzymatically by purine nucleoside phosphorylase (PNPase) to the products ribose 1-phosphate and 2-amino-6-mercapto-7-methylpurine. An application note describes the dephosphorylation reaction of phytic acid using phytase and alkaline phosphatase (sold separately), and subsequent phosphate detection using the reagents provided in K-PHOS.

This method is suitable for analysis using spectrophotometer and auto-analyser.

Note for Content: The number of manual tests per kit can be doubled if all volumes are halved.

View Documents

Analyte Phosphate
Application Examples Processed foods and drinks, bakery products, dairy products, waste water samples, cosmetics, pharmaceuticals and other materials (e.g. biological cultures, etc.).
Brand Megazyme
Limit of Detection 0.16 mg/L
Linear Range 0.1 to 10 μg of phosphate per assay
Package Dimensions 10.00 IN x 5.00 IN x 2.00 IN
Package Weight 0.47 LB
Platform Spectrophotometer, Auto-analyser
Reaction Time ~ 20 min
Result Type UV Absorbance
Signal Response Increase
Storage Conditions

Storage Temperature: Short term stability: 2-8°C, Long term stability: See individual component labels

Stability: > 2 years under recommended storage conditions

Wavelength 360 nm