Hydrogen Peroxide Assay Kit (Megaplex Red)

1000 assays (microplate) / 100 assays (manual) / 400 assays (auto-analyser)

Item No.  K-MRH2O2

  • Simple format
  • Very competitive price (cost per test)
  • Ability to run in fluorescence or absorbance mode
  • User friendly - Detailed protocol provided for the creation of calibration curve and the calculation of concentration in fluorescence mode. Single point standard for quicker and simpler analysis in absorbance mode.
  • Mega-Calc™ software tool is available from our website for hassle-free raw data processing
  • Suitable for manual, microplate and auto-analyser formats
  • All reagents stable for > 2 years
This item has shipping restrictions.
In stock
Out of stock
Expect availability 10/27/2021
Minimum quantity required: 1
This item must be ordered in multiples of one case. Quantity per case: 1

The hydrogen peroxide Assay Kit provides a simple robust method for the measurement of hydrogen peroxide. This kit utilizes a highly sensitive Megaplex Red probe which in the presence of horseradish peroxidase (HRP), allows for the measurement of H2O2 in a sample. Upon oxidation of the probe by HRP, a coloured product (resorufin) is formed that can be measured in fluorescence mode using excitation in the range 530-560 nm and emission at ~ 590 nm or colourimetrically at 570 nm. The excitation and emission maxima of Megaplex Red are 571 nm and 585 nm respectively.

Fluorometric/UV method for the determination of H2O2 content in a variety of samples.

View Documents

Analyte Hydrogen Peroxide
Application Examples Food and beverage samples such as milk or milk based products, wine, fruit juices, paper (and cardboard), waste treatment samples and other materials (e.g. biological cultures etc.).
Brand Megazyme
Limit of Detection (Fluorescence) 0.0034 ug/mL,
(Absorbance) 0.25 ug/mL
Linear Range (Fluorescence) 0.0034-0.17 µg of H2O2 per assay (0.1-5 µM in-assay),
(Absorbance) 0.034-0.68 µg of H2O2 per assay (1-20 µM in-assay)
Package Dimensions 10.00 IN x 5.00 IN x 2.00 IN
Package Weight 0.65 LB
Platform Spectrophotometer, Microplate, Auto-analyser
Reaction Time (Fluorescence) 30 min,
(Absorbance) 15 min
Reproducibility (Fluorescence) ~ 8%,
(Absorbance) ~ 8%
Result Type UV Absorbance, Fluorescence
Signal Response Increase
Storage Conditions

Storage Temperature: Short term stability: 2-8°C, Long term stability: See individual component labels

Stability: > 2 years under recommended storage conditions

Wavelength (Fluorescence) 530-560 (ex), ~ 590 (em)
(Absorbance) 570