Xylanase Assay Kit (XylX6 Method)
Item No. K-XYLX6-2V
- Very cost effective
- All reagents stable for > 4 years
- Completely specific for endo-1,4-β-xylanase
- Generally applicable and highly sensitive
- Simple format. Well suited to automation
- Excellent reproducibility
- Standard included
The XylX6 assay reagent for the measurement of endo-xylanase (endo-1,4-β-xylanase) contains two components; 1) 4,6-O-(3-Ketobutylidene)-4-nitrophenyl-β-D-45-glucosyl-xylopentaoside and 2) β-xylosidase. The ketone blocking group prevents any hydrolytic action by the β-xylosidase or other exo-acting glycosidases on the XylX6 substrate. Incubation with an endo-xylanase generates a non-blocked colourimetric oligosaccharide that is rapidly hydrolysed by the ancillary β-xylosidase. The rate of formation of 4-nitrophenol is therefore directly related to the hydrolysis of XylX6 by the endo-xylanase. The reaction is terminated and the phenolate colour is developed on addition of Tris buffer solution (pH = 10.0).
Note that standard curves relating the absorbance obtained using the XylX6 assay to endo-xylanase activity on the native substrates, wheat arabinoxylan and beechwood xylan, are provided in the Supporting Information file in the Documents section.
| Specifications | ||
|---|---|---|
| Brand | Megazyme | |
| Analyte | endo-1,4-α-Xylanase | |
| Platform | Spectrophotometer, Auto-analyser | |
| Result Type | UV Absorbance | |
| Approvals | Novel method | |
| Limit of Detection | 5.3 x 10-4 U/mL | |
| Storage Conditions |
Storage Temperature: Short term stability: 2-8°C, Long term stability: See individual component labels Stability: > 2 years under recommended storage conditions |
|
| Wavelength | 400 nm, 405 nm | |
| Application Examples | Fermentation broths, industrial enzyme preparations, animal feed, biofuels research, barley malt analysis. | |
| Signal Response | Increase | |
| Total Assay Time | 10 min | |
| Reproducibility | ~ 3% | |
| Package Dimensions | 10.00 in x 5.00 in x 2.00 in | |
| Package Weight | 0.25 lb | |