Xylanase Assay Kit (XylX6 Method)

Item No.  K-XYLX6-2V

  • Very cost effective
  • All reagents stable for > 4 years
  • Completely specific for endo-1,4-β-xylanase
  • Generally applicable and highly sensitive
  • Simple format. Well suited to automation
  • Excellent reproducibility
  • Standard included
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The XylX6 assay reagent for the measurement of endo-xylanase (endo-1,4-β-xylanase) contains two components; 1) 4,6-O-(3-Ketobutylidene)-4-nitrophenyl-β-D-45-glucosyl-xylopentaoside and 2) β-xylosidase. The ketone blocking group prevents any hydrolytic action by the β-xylosidase or other exo-acting glycosidases on the XylX6 substrate. Incubation with an endo-xylanase generates a non-blocked colourimetric oligosaccharide that is rapidly hydrolysed by the ancillary β-xylosidase. The rate of formation of 4-nitrophenol is therefore directly related to the hydrolysis of XylX6 by the endo-xylanase. The reaction is terminated and the phenolate colour is developed on addition of Tris buffer solution (pH = 10.0).

Note that standard curves relating the absorbance obtained using the XylX6 assay to endo-xylanase activity on the native substrates, wheat arabinoxylan and beechwood xylan, are provided in the Supporting Information file in the Documents section.

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Specifications
Brand Megazyme
Analyte endo-1,4-α-Xylanase
Platform Spectrophotometer, Auto-analyser
Result Type UV Absorbance
Approvals Novel method
Limit of Detection 5.3 x 10-4 U/mL
Storage Conditions

Storage Temperature: Short term stability: 2-8°C, Long term stability: See individual component labels

Stability: > 2 years under recommended storage conditions

Wavelength 400 nm, 405  nm
Application Examples Fermentation broths, industrial enzyme preparations, animal feed, biofuels research, barley malt analysis.
Signal Response Increase
Total Assay Time 10 min
Reproducibility ~ 3%
Package Dimensions 10.00 in x 5.00 in x 2.00 in
Package Weight 0.25 lb

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