Pullulanase/Limit-Dextrinase Assay Kit (PullG6 Method)
100/200 assays per kit
Item No. 700004329 | Catalog No. K-PULLG6
- High sensitivity
- Suitable for manual and auto-analyser formats
- No transglycosylation interference
- Very cost effective
- All reagents stable for > 1 year after preparation
- Very specific
- Simple format
- Standard included
PullG6 assay for the measurement of pullulanase employs a water soluble defined substrate, namely 4,6-O-benzylidene-4-nitrophenyl-63-α-D-maltotriosyl-maltotriose (BPNPG3G3), coupled with the ancillary enzymes α-glucosidase and β-glucosidase. Upon hydrolysis of the substrate at the 1,6-α-linkage by pullulanase or limit-dextrinase, the released 4-nitrophenyl-β-maltotrioside is immediately hydrolysed to glucose and 4-nitrophenol by the concerted action of the α-glucosidase and β-glucosidase enzymes in the reagent mixture. The reaction is terminated and phenolate ions are developed by addition of dilute alkali. The absorbance is read at 400 nm and the value obtained correlates directly with pullulanase activity.
| Specifications | ||
|---|---|---|
| Brand | Megazyme | |
| Analyte | Pullulanase, Limit-dextrinase | |
| Platform | Spectrophotometer | |
| Result Type | UV Absorbance | |
| Approvals | Novel method | |
| Limit of Detection | 0.18 U/mL for pullulanase preparations (50-fold dilution), 0.01 U/g for limit dextrinase in milled malt | |
| Storage Conditions |
Storage Temperature: Short term stability: 2-8°C, Long term stability: See individual component labels Stability: > 2 years under recommended storage conditions |
|
| Wavelength | 400 nm | |
| Application Examples | Assay of microbial pullulanase preparations. Measurement of limit-dextrinase in malt extracts. | |
| Signal Response | Increase | |
| Total Assay Time |
Approx. 10 min (pullanase); Approx. 30 min (limit-dextrinase) |
|
| Reproducibility | ~ 3% | |
| Package Dimensions | 10.00 in x 5.00 in x 2.00 in | |
| Package Weight | 0.50 lb | |
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