Pullulanase/Limit-Dextrinase Assay Kit (PullG6 Method)

100/200 assays per kit

Item No.  K-PULLG6

  • High sensitivity
  • Suitable for manual and auto-analyser formats
  • No transglycosylation interference
  • Very cost effective
  • All reagents stable for > 1 year after preparation
  • Very specific
  • Simple format
  • Standard included
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PullG6 assay for the measurement of pullulanase employs a water soluble defined substrate, namely 4,6-O-benzylidene-4-nitrophenyl-63-α-D-maltotriosyl-maltotriose (BPNPG3G3), coupled with the ancillary enzymes α-glucosidase and β-glucosidase. Upon hydrolysis of the substrate at the 1,6-α-linkage by pullulanase or limit-dextrinase, the released 4-nitrophenyl-β-maltotrioside is immediately hydrolysed to glucose and 4-nitrophenol by the concerted action of the α-glucosidase and β-glucosidase enzymes in the reagent mixture. The reaction is terminated and phenolate ions are developed by addition of dilute alkali. The absorbance is read at 400 nm and the value obtained correlates directly with pullulanase activity.

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Specifications
Brand Megazyme
Analyte Pullulanase, Limit-dextrinase
Platform Spectrophotometer
Result Type UV Absorbance
Approvals Novel method
Limit of Detection 0.18 U/mL for pullulanase preparations (50-fold dilution), 0.01 U/g for limit dextrinase in milled malt
Storage Conditions

Storage Temperature: Short term stability: 2-8°C, Long term stability: See individual component labels

Stability: > 2 years under recommended storage conditions

Wavelength 400 nm
Application Examples Assay of microbial pullulanase preparations. Measurement of limit-dextrinase in malt extracts.
Signal Response Increase
Total Assay Time Approx. 10 min (pullanase);
Approx. 30 min (limit-dextrinase)
Reproducibility ~ 3%
Package Weight 0.50 lb

Documents

To access the Certificate of Analysis (COA), please use our COA Search.

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