Malt β-Glucanase/Lichenase Assay Kit (MBG4 Method)

100 assays (manual) / 400 assays (auto-analyser)

Item No.  K-MBG4

  • Very cost effective
  • All reagents stable for > 2 years
  • Specific for endo-1,3:1,4-β-glucanase/lichenase
  • Simple, convenient, rapid assay
  • Well suited to automation
  • Malt flour standard and lichenase standard included
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$347.00 each
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Minimum quantity required: 1
This item must be ordered in multiples of one case. Quantity per case: 1

The MBG4 reagent contains a single substrate, namely 4,6-O-benzylidene-2-chloro-4-nitrophenyl-β-(31-β-D-cellotriosyl-glucoside) (BCNPBG4). The benzylidene acetal group prevents any hydrolytic action by exo-acting hydrolytic enzymes such as β-glucosidase or cellobiohydrolase.

Mixed linkage β-glucanase (endo-1,3:1,4-β-glucanase) / lichenase (EC 3.2.1.73) acts specifically to release 2-chloro-4-nitrophenol (CNP) from this substrate. The rate of release of CNP is directly related to the β-glucanase/lichenase activity in a sample. The reaction is terminated and the phenolate colour is developed on addition of Tris buffer solution (pH = 10.0).

Note that the substrate is not hydrolysed by β-glucosidase or cellobiohydrolase. The substrate can be hydrolysed by certain endo-cellulases (e.g. Trichoderma sp.) but this does not result in an increase in absorbance.

Content:

  • (Malt β-glucanase) 100 assays (manual) / 400 (auto-analyser) or
  • (Lichenase) 100 / 200 assays (manual) / 330 (auto-analyser)

View Documents

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Specifications
Brand Megazyme
Analyte Lichenase
Platform Spectrophotometer, Auto-analyser
Result Type UV Absorbance
Approvals Novel method
Limit of Detection (Malt β-glucanase) 4.3 x 10-4 U/mL Or (Lichenase) 9.1 x 10-5 U/mL
Storage Conditions

Storage Temperature: Short term stability: 2-8°C, Long term stability: See individual component labels

Stability: > 2 years under recommended storage conditions

Wavelength 400 nm
Application Examples Crude malt extracts, industrial enzyme preparations.
Signal Response Increase
Total Assay Time Approx. 20 min (Malt β-glucanase);
Approx. 10 min (Lichenase)
Reproducibility ~ 3%
Package Dimensions 10.00 in x 5.00 in x 2.00 in
Package Weight 0.39 lb

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