Malt β-Glucanase/Lichenase Assay Kit (MBG4 Method)

100 assays (manual) / 400 assays (auto-analyser)

Item No.  K-MBG4

  • Very cost effective
  • All reagents stable for > 2 years
  • Specific for endo-1,3:1,4-β-glucanase/lichenase
  • Simple, convenient, rapid assay
  • Well suited to automation
  • Malt flour standard and lichenase standard included
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$388.64 each
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Expect availability in 2-4 weeks
Qty:
Minimum quantity required: 1
This item must be ordered in multiples of one case. Quantity per case: 1

The MBG4 reagent contains a single substrate, namely 4,6-O-benzylidene-2-chloro-4-nitrophenyl-β-(31-β-D-cellotriosyl-glucoside) (BCNPBG4). The benzylidene acetal group prevents any hydrolytic action by exo-acting hydrolytic enzymes such as β-glucosidase or cellobiohydrolase.

Mixed linkage β-glucanase (endo-1,3:1,4-β-glucanase) / lichenase (EC 3.2.1.73) acts specifically to release 2-chloro-4-nitrophenol (CNP) from this substrate. The rate of release of CNP is directly related to the β-glucanase/lichenase activity in a sample. The reaction is terminated and the phenolate colour is developed on addition of Tris buffer solution (pH = 10.0).

Note that the substrate is not hydrolysed by β-glucosidase or cellobiohydrolase. The substrate can be hydrolysed by certain endo-cellulases (e.g. Trichoderma sp.) but this does not result in an increase in absorbance.

Content:

  • (Malt β-glucanase) 100 assays (manual) / 400 (auto-analyser) or
  • (Lichenase) 100 / 200 assays (manual) / 330 (auto-analyser)

View Documents

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Specifications
Brand Megazyme
Analyte Lichenase
Platform Spectrophotometer, Auto-analyser
Result Type UV Absorbance
Approvals Novel method
Limit of Detection (Malt β-glucanase) 4.3 x 10-4 U/mL Or (Lichenase) 9.1 x 10-5 U/mL
Storage Conditions

Storage Temperature: Short term stability: 2-8°C, Long term stability: See individual component labels

Stability: > 2 years under recommended storage conditions

Wavelength 400 nm
Application Examples Crude malt extracts, industrial enzyme preparations.
Signal Response Increase
Total Assay Time Approx. 20 min (Malt β-glucanase);
Approx. 10 min (Lichenase)
Reproducibility ~ 3%
Package Weight 0.39 lb

Documents

To access the Certificate of Analysis (COA), please use our COA Search.

Malt β-Glucanase/Lichenase Assay Kit (MBG4 Method) - Bulgarian - Bulgaria Malt β-Glucanase/Lichenase Assay Kit (MBG4 Method) - Chinese Simplified Malt β-Glucanase/Lichenase Assay Kit (MBG4 Method) - Croatian - Croatia Malt β-Glucanase/Lichenase Assay Kit (MBG4 Method) - Czech - Czech Republic Malt β-Glucanase/Lichenase Assay Kit (MBG4 Method) - Danish - Denmark Malt β-Glucanase/Lichenase Assay Kit (MBG4 Method) - Dutch - Netherlands Malt β-Glucanase/Lichenase Assay Kit (MBG4 Method) - English - Australia Malt β-Glucanase/Lichenase Assay Kit (MBG4 Method) - English - Australia Malt β-Glucanase/Lichenase Assay Kit (MBG4 Method) - English - EU/UK Malt β-Glucanase/Lichenase Assay Kit (MBG4 Method) - English - Ireland Malt β-Glucanase/Lichenase Assay Kit (MBG4 Method) - English - New Zealand Malt β-Glucanase/Lichenase Assay Kit (MBG4 Method) - English - US Malt β-Glucanase/Lichenase Assay Kit (MBG4 Method) - Estonian - Estonia Malt β-Glucanase/Lichenase Assay Kit (MBG4 Method) - Finnish - Finland Malt β-Glucanase/Lichenase Assay Kit (MBG4 Method) - French Malt β-Glucanase/Lichenase Assay Kit (MBG4 Method) - German Malt β-Glucanase/Lichenase Assay Kit (MBG4 Method) - Greek - Greece Malt β-Glucanase/Lichenase Assay Kit (MBG4 Method) - Hungarian - Hungary Malt β-Glucanase/Lichenase Assay Kit (MBG4 Method) - Icelandic - Iceland Malt β-Glucanase/Lichenase Assay Kit (MBG4 Method) - Italian - Italy Malt β-Glucanase/Lichenase Assay Kit (MBG4 Method) - Japanese - Japan Malt β-Glucanase/Lichenase Assay Kit (MBG4 Method) - Latvian - Latvia Malt β-Glucanase/Lichenase Assay Kit (MBG4 Method) - Lithuanian - Lithuania Malt β-Glucanase/Lichenase Assay Kit (MBG4 Method) - Maltese - Malta Malt β-Glucanase/Lichenase Assay Kit (MBG4 Method) - Polish - Poland Malt β-Glucanase/Lichenase Assay Kit (MBG4 Method) - Portuguese Malt β-Glucanase/Lichenase Assay Kit (MBG4 Method) - Romanian - Romania Malt β-Glucanase/Lichenase Assay Kit (MBG4 Method) - Slovak - Slovakia Malt β-Glucanase/Lichenase Assay Kit (MBG4 Method) - Slovene - Slovenia Malt β-Glucanase/Lichenase Assay Kit (MBG4 Method) - Spanish Malt β-Glucanase/Lichenase Assay Kit (MBG4 Method) - Swedish - Sweden

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