Malt β-Glucanase/Lichenase Assay Kit (MBG4 Method)
100 assays (manual) / 400 assays (auto-analyser)
Item No. K-MBG4
- Very cost effective
- All reagents stable for > 2 years
- Specific for endo-1,3:1,4-β-glucanase/lichenase
- Simple, convenient, rapid assay
- Well suited to automation
- Malt flour standard and lichenase standard included
The MBG4 reagent contains a single substrate, namely 4,6-O-benzylidene-2-chloro-4-nitrophenyl-β-(31-β-D-cellotriosyl-glucoside) (BCNPBG4). The benzylidene acetal group prevents any hydrolytic action by exo-acting hydrolytic enzymes such as β-glucosidase or cellobiohydrolase.
Mixed linkage β-glucanase (endo-1,3:1,4-β-glucanase) / lichenase (EC 3.2.1.73) acts specifically to release 2-chloro-4-nitrophenol (CNP) from this substrate. The rate of release of CNP is directly related to the β-glucanase/lichenase activity in a sample. The reaction is terminated and the phenolate colour is developed on addition of Tris buffer solution (pH = 10.0).
Note that the substrate is not hydrolysed by β-glucosidase or cellobiohydrolase. The substrate can be hydrolysed by certain endo-cellulases (e.g. Trichoderma sp.) but this does not result in an increase in absorbance.
Content:
- (Malt β-glucanase) 100 assays (manual) / 400 (auto-analyser) or
- (Lichenase) 100 / 200 assays (manual) / 330 (auto-analyser)
| Specifications | ||
|---|---|---|
| Brand | Megazyme | |
| Analyte | Lichenase | |
| Platform | Spectrophotometer, Auto-analyser | |
| Result Type | UV Absorbance | |
| Approvals | Novel method | |
| Limit of Detection | (Malt β-glucanase) 4.3 x 10-4 U/mL Or (Lichenase) 9.1 x 10-5 U/mL | |
| Storage Conditions |
Storage Temperature: Short term stability: 2-8°C, Long term stability: See individual component labels Stability: > 2 years under recommended storage conditions |
|
| Wavelength | 400 nm | |
| Application Examples | Crude malt extracts, industrial enzyme preparations. | |
| Signal Response | Increase | |
| Total Assay Time |
Approx. 20 min (Malt β-glucanase); Approx. 10 min (Lichenase) |
|
| Reproducibility | ~ 3% | |
| Package Weight | 0.39 lb | |