Cellulase Assay Kit (CellG5 Method)
Item No. K-CELLG5-4V
- Very cost effective
- All reagents stable for > 4 years
- Completely specific for cellulase (endo-1,4-glucanase)
- Generally applicable and highly sensitive
- Simple format. Well suited to automation
- Standard included
Cellulase Activity Assay Kit.
The CellG5 assay reagent for the measurement of endo-cellulase (endo-1,4-β-glucanase) contains two components; 1) 4,6-O-(3-Ketobutylidene)-4-nitrophenyl-β-D-cellopentaoside (BPNPG5) and 2) thermostable β-glucosidase. The ketone blocking group prevents any hydrolytic action by the β-glucosidase on BPNPG5. Incubation with an endo-cellulase generates a non-blocked colourimetric oligosaccharide that is rapidly hydrolysed by the ancillary β-glucosidase. The rate of formation of 4-nitrophenol is therefore directly related to the hydrolysis of BPNPG5 by the endo-cellulase. The reaction is terminated and the phenolate colour is developed on addition of Tris buffer solution (pH 9.0).
The CellG5 assay represents a huge step forward in the methodology for the measurement of cellulase that traditionally relied on substrates such as CM-cellulose, Avicel, cellooligosaccharides, filter paper or dyed polysaccharides including CMC Congo red or cellulose azure.
| Specifications | ||
|---|---|---|
| Brand | Megazyme | |
| Analyte | endo-Cellulase | |
| Platform | Spectrophotometer, Auto-analyser | |
| Result Type | UV Absorbance | |
| Approvals | Novel method | |
| Limit of Detection | 1.2 x 10-3 U/mL | |
| Storage Conditions |
Storage Temperature: Short term stability: 2-8°C, Long term stability: See individual component labels Stability: > 2 years under recommended storage conditions |
|
| Wavelength | 400 nm | |
| Application Examples | Fermentation broths, industrial enzyme preparations and biofuels research. | |
| Signal Response | Increase | |
| Total Assay Time | 10 min | |
| Reproducibility | ~ 3% | |
| Package Weight | 0.44 lb | |
Documents
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