Cellulase Assay Kit (CellG5 Method)

Item No.  K-CELLG5-4V

  • Very cost effective
  • All reagents stable for > 4 years
  • Completely specific for cellulase (endo-1,4-glucanase)
  • Generally applicable and highly sensitive
  • Simple format. Well suited to automation
  • Standard included
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Cellulase Activity Assay Kit.

The CellG5 assay reagent for the measurement of endo-cellulase (endo-1,4-β-glucanase) contains two components; 1) 4,6-O-(3-Ketobutylidene)-4-nitrophenyl-β-D-cellopentaoside (BPNPG5) and 2) thermostable β-glucosidase. The ketone blocking group prevents any hydrolytic action by the β-glucosidase on BPNPG5.  Incubation with an endo-cellulase generates a non-blocked colourimetric oligosaccharide that is rapidly hydrolysed by the ancillary β-glucosidase. The rate of formation of 4-nitrophenol is therefore directly related to the hydrolysis of BPNPG5 by the endo-cellulase. The reaction is terminated and the phenolate colour is developed on addition of Tris buffer solution (pH 9.0).

The CellG5 assay represents a huge step forward in the methodology for the measurement of cellulase that traditionally relied on substrates such as CM-cellulose, Avicel, cellooligosaccharides, filter paper or dyed polysaccharides including CMC Congo red or cellulose azure.

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Specifications
Brand Megazyme
Analyte endo-Cellulase
Platform Spectrophotometer, Auto-analyser
Result Type UV Absorbance
Approvals Novel method
Limit of Detection 1.2 x 10-3 U/mL
Storage Conditions

Storage Temperature: Short term stability: 2-8°C, Long term stability: See individual component labels

Stability: > 2 years under recommended storage conditions

Wavelength 400 nm
Application Examples Fermentation broths, industrial enzyme preparations and biofuels research.
Signal Response Increase
Total Assay Time 10 min
Reproducibility ~ 3%
Package Weight 0.44 lb

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