Trehalase (prokaryote)

8,400 Units

Item No.  E-TREH

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High purity Trehalase (prokaryote) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

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Specifications
Application Examples Applications established for the measurement of trehalose in the food, fermentation, wine, beverage and dairy industries.
Brand Megazyme
CAS Number 9025-52-9
CAZy Family GH37
Concentration ~ 4200 U/mL
EC Number 3.2.1.28 
Enzyme Other Activities
Expression Recombinant from a Prokaryotic source
Formulation In 3.2 M ammonium sulphate
Molecular Weight 63636
Package Dimensions 0.70 IN x 0.70 IN x 1.60 IN
Package Weight 0.53 LB
pH Optima 5.5-5.9
Physical Form Suspension
Source Prokaryote
Specificity Catalyses the reaction: Trehalose + H2O = β-D-glucose + α-D-glucose
Specific Activity ~ 180 U/mg (40°C, pH 5.5 on trehalose)
Storage Conditions

Storage Temperature: 2-8°C

Stability: > 4 years at 4°C

Synonyms alpha,alpha-trehalase; alpha,alpha-trehalose glucohydrolase
Temperature Optima (°C) 40
Unit Definition One Unit of trehalase activity is defined as the amount of enzyme required to release two μmoles of D-glucose per minute from trehalose (5 mg/mL) in sodium maleate buffer (100 mM), pH 5.5 at 40°C.

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