Succinyl-CoA synthetase (prokaryote)

550 Units

SKU No.  700004239  |  Catalog No.  E-SCOAS

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High purity recombinant Succinyl-CoA synthetase (prokaryote) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

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Specifications
BrandMegazyme
EC Number6.2.1.5
EnzymeOther Activities
Storage Conditions

Storage Temperature: 2-8°C

Stability: > 1 year under recommended storage conditions

Application ExamplesApplications for the measurement of succinic acid in the clinical chemistry, food, wine and dairy industries.
FormulationIn 3.2 M ammonium sulphate
Physical FormSuspension
CAS Number9080-33-5
Concentration~ 220
Synonymssuccinate-CoA ligase (ADP-forming); succinate:CoA ligase (ADP-forming)
SourceProkaryote
Molecular Weight30,843,
41,393
ExpressionRecombinant from a Prokaryotic source
SpecificityCatalyses the reaction: ATP + succinate + CoA = ADP + phosphate + succinyl-CoA.
Specific Activity~ 9 U/mg (25°C, pH 8.4 on succinic acid)
Unit DefinitionOne Unit of Succinyl-CoA synthetase activity is defined as the amount of enzyme required to release one μmole of succinyl CoA from succinic acid (5.8 mM) per minute in the presence of NADH and Coenzyme A in Glycylglycine buffer (34 mM), pH 8.4 at 25°C.
Temperature Optima (°C)25
pH Optima8.4
Package Dimensions0.70 in x 0.70 in x 1.60 in
Package Weight0.01 lb