Succinyl-CoA synthetase (prokaryote)
550 Units
Item No. E-SCOAS
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High purity recombinant Succinyl-CoA synthetase (prokaryote) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
| Specifications | ||
|---|---|---|
| Brand | Megazyme | |
| EC Number | 6.2.1.5 | |
| Enzyme | Other Activities | |
| Storage Conditions |
Storage Temperature: 2-8°C |
|
| Application Examples | Applications for the measurement of succinic acid in the clinical chemistry, food, wine and dairy industries. | |
| Formulation | In 3.2 M ammonium sulphate | |
| Physical Form | Suspension | |
| CAS Number | 9080-33-5 | |
| Concentration | ~ 220 U/mL | |
| Synonyms | succinate-CoA ligase (ADP-forming); succinate:CoA ligase (ADP-forming) | |
| Source | Prokaryote | |
| Molecular Weight |
30,843, 41,393 |
|
| Expression | Recombinant from a Prokaryotic source | |
| Specificity | Catalyses the reaction: ATP + succinate + CoA = ADP + phosphate + succinyl-CoA. | |
| Specific Activity | ~ 7 U/mg (25°C, pH 8.4 on succinic acid) | |
| Unit Definition | One Unit of Succinyl-CoA synthetase activity is defined as the amount of enzyme required to release one μmole of succinyl CoA from succinic acid (5.8 mM) per minute in the presence of NADH and Coenzyme A in Glycylglycine buffer (34 mM), pH 8.4 at 25°C. | |
| Temperature Optima (°C) | 25 | |
| pH Optima | 8.0-8.4 | |
| Package Weight | 0.01 | |