Succinyl-CoA synthetase (prokaryote) 

550 Units

Item No.  E-SCOAS

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High purity recombinant Succinyl-CoA synthetase (prokaryote) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

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Brand Megazyme
EC Number
Enzyme Other Activities
Storage Conditions

Storage Temperature: 2-8°C

Application Examples Applications for the measurement of succinic acid in the clinical chemistry, food, wine and dairy industries.
Formulation In 3.2 M ammonium sulphate
Physical Form Suspension
CAS Number 9080-33-5 
Concentration ~ 220 U/mL
Synonyms succinate-CoA ligase (ADP-forming); succinate:CoA ligase (ADP-forming)
Source Prokaryote
Molecular Weight 30,843,
Expression Recombinant from a Prokaryotic source
Specificity Catalyses the reaction: ATP + succinate + CoA = ADP + phosphate + succinyl-CoA.
Specific Activity ~ 7 U/mg (25°C, pH 8.4 on succinic acid)
Unit Definition One Unit of Succinyl-CoA synthetase activity is defined as the amount of enzyme required to release one μmole of succinyl CoA from succinic acid (5.8 mM) per minute in the presence of NADH and Coenzyme A in Glycylglycine buffer (34 mM), pH 8.4 at 25°C. 
Temperature Optima (°C) 25
pH Optima 8.0-8.4
Package Weight 0.01