Pullulanase M2 (Bacillus licheniformis)

2,000 Units

SKU No.  700004236  |  Catalog No.  E-PULBL

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Minimum quantity required: 1
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High purity Pullulanase M2 (Bacillus licheniformis) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

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Specifications
BrandMegazyme
EC Number3.2.1.41
EnzymePullulanase, Limit-dextrinase
Storage Conditions

Storage Temperature: 2-8°C

Stability: > 1 year under recommended storage conditions

Application ExamplesApplications in the cereals, food and feeds industries particularly in starch saccharification and production of high glucose or maltose syrups.
FormulationIn 3.2 M ammonium sulphate
Physical FormSuspension
CAS Number9075-68-7
CAZy FamilyGH13
Concentration~ 1000 U/mL
Synonymspullulanase; pullulan 6-alpha-glucanohydrolase
SourceBacillus licheniformis
Molecular Weight113000
ExpressionPurified from Bacillus licheniformis
SpecificityHydrolysis of (1,6)-α-D-glucosidic linkages in pullulan, amylopectin and glycogen, and in the α and β-limit dextrins of amylopectin and glycogen.
Specific Activity~ 78 U/mg (40°C, pH 5.0 on pullulan)
Unit DefinitionOne Unit of pullulanase M2 activity is defined as the amount of enzyme required to release one µmole of glucose reducing-sugar-equivalents per minute from pullulan (10 mg/mL) in sodium acetate buffer (100 mM), pH 5.0 at 40°C.
Temperature Optima (°C)55
pH Optima5
Package Dimensions0.80 in x 0.80 in x 1.90 in
Package Weight0.03 lb