Pullulanase M1 (Klebsiella planticola)
700 Units
Item No. E-PULKP
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High purity Pullulanase M1 (Klebsiella planticola) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
Recommended pullulanase for research on starch structure.
| Specifications | ||
|---|---|---|
| Brand | Megazyme | |
| EC Number | 3.2.1.41 | |
| Enzyme | Pullulanase, Limit-dextrinase | |
| Storage Conditions |
Storage Temperature: 2-8°C Stability: > 4 years at 4°C |
|
| Application Examples | Applications in the cereals, food and feeds industries particularly in starch saccharification and production of high glucose or maltose syrups. | |
| Formulation | In 3.2 M ammonium sulphate | |
| Physical Form | Suspension | |
| CAS Number | 9075-68-7 | |
| CAZy Family | GH13 | |
| Concentration | ~ 650 | |
| Synonyms | pullulanase; pullulan 6-alpha-glucanohydrolase | |
| Source | Klebsiella planticola | |
| Molecular Weight | 109000 | |
| Expression | Purified from Klebsiella planticola | |
| Specificity | Hydrolysis of (1,6)-α-D-glucosidic linkages in pullulan, amylopectin and glycogen, and in the α- and β-limit dextrins of amylopectin and glycogen. | |
| Specific Activity | ~ 30 U/mg (40°C, pH 5.0 on pullulan) | |
| Unit Definition | One Unit of pullulanase activity is defined as the amount of enzyme required to release one µmole of glucose reducing-sugar-equivalents per minute from pullulan (5 mg/mL) in sodium acetate buffer (100 mM), pH 5.0 at 40°C. | |
| Temperature Optima (°C) | 50 | |
| pH Optima | 5.0-5.4 | |
| Package Weight | 0.03 lb | |
Documents
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