Pullulanase M1 (Klebsiella planticola)

700 Units

Item No.  E-PULKP

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High purity Pullulanase M1 (Klebsiella planticola) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

Recommended pullulanase for research on starch structure.

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Specifications
Brand Megazyme
EC Number 3.2.1.41 
Enzyme Pullulanase, Limit-dextrinase
Storage Conditions

Storage Temperature: 2-8°C

Stability: > 4 years at 4°C

Application Examples Applications in the cereals, food and feeds industries particularly in starch saccharification and production of high glucose or maltose syrups.
Formulation In 3.2 M ammonium sulphate
Physical Form Suspension
CAS Number 9075-68-7 
CAZy Family GH13
Concentration ~ 650 U/mL
Synonyms pullulanase; pullulan 6-alpha-glucanohydrolase
Source Klebsiella planticola
Molecular Weight 109000
Expression Purified from Klebsiella planticola
Specificity Hydrolysis of (1,6)-α-D-glucosidic linkages in pullulan, amylopectin and glycogen, and in the α- and β-limit dextrins of amylopectin and glycogen.
Specific Activity ~ 30 U/mg (40°C, pH 5.0 on pullulan)
Unit Definition One Unit of pullulanase activity is defined as the amount of enzyme required to release one µmole of glucose reducing-sugar-equivalents per minute from pullulan (5 mg/mL) in sodium acetate buffer (100 mM), pH 5.0 at 40°C.
Temperature Optima (°C) 50
pH Optima 5.0-5.4
Package Weight 0.03 lb

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