Phosphomannose isomerase (Escherichia coli)

1,000 Units

SKU No.  700004234  |  Catalog No.  E-PMIEC

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Minimum quantity required: 1
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High purity recombinant Phosphomannose isomerase (Escherichia coli) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

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Specifications
BrandMegazyme
EC Number5.3.1.8
EnzymeIsomerase
Storage Conditions

Storage Temperature: 2-8°C

Stability: > 1 year under recommended storage conditions

Application ExamplesApplications for the measurement of D-mannose in carbohydrate research and in the food and feeds, fermentation, wine, beverage and dairy industries.
FormulationIn 3.2 M ammonium sulphate
Physical FormSuspension
CAS Number9023-88-5
Concentration~ 500
Synonymsmannose-6-phosphate isomerase; D-mannose-6-phosphate aldose-ketose-isomerase
SourceEscherichia coli
Molecular Weight43915
ExpressionRecombinant from Escherichia coli
SpecificityCatalyses the reaction: D-Mannose 6-phosphate = D-fructose 6-phosphate.
Specific Activity~ 89 U/mg (25°C, pH 7.6 on mannose 6-phosphate)
Unit DefinitionOne Unit of phosphomannose isomerase activity is defined as the amount of enzyme required to release one µmole of fructose 6-phosphate from mannose 6-phosphate (3.14 mM) per minute in the presence of NAD+ at pH 7.6 and 25°C.
Temperature Optima (°C)40
pH Optima7.6
Package Dimensions0.70 in x 0.70 in x 1.60 in
Package Weight0.09 lb