Phosphoglucose isomerase (Escherichia coli)

Item No.  E-PGIEC-50KU

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High purity Phosphoglucose isomerase (Escherichia coli) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

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Specifications
Brand Megazyme
EC Number 5.3.1.9 
Enzyme Isomerase
Storage Conditions

Storage Temperature: 2-8°C

Stability: > 4 years at 4°C

Application Examples Applications for the measurement of D-fructose in carbohydrate research and in the food and feeds, fermentation, wine, beverage and dairy industries.
Formulation In 3.2 M ammonium sulphate
Physical Form Suspension
CAS Number 9001-41-6 
Concentration E-PGIEC-10KU: ~ 1,000 U/mL.
E-PGIEC-50KU: ~ 10,000 U/mL.
Synonyms glucose-6-phosphate isomerise; D-glucose-6-phosphate aldose-ketose-isomerase
Source Escherichia coli
Molecular Weight 62600
Expression Recombinant from Escherichia coli
Specificity Catalyses the reaction: D-Glucose 6-phosphate = D-fructose 6-phosphate.
Specific Activity ~ 500 U/mg of protein (25°C, pH 7.6 on fructose 6-phosphate);
~ 650 U/mg of protein (40°C, pH 7.6 on fructose 6-phosphate)
Unit Definition One unit of phosphoglucose isomerase activity is the amount of enzyme required to convert one µmole of D-fructose 6-phosphate to D-glucose 6-phosphate per min at pH 7.6.
Temperature Optima (°C) 40
pH Optima 7.5-7.9
Package Weight 0.04 lb

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