Phosphoglucose isomerase (Escherichia coli)
Item No. E-PGIEC-50KU
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High purity Phosphoglucose isomerase (Escherichia coli) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
| Specifications | ||
|---|---|---|
| Brand | Megazyme | |
| EC Number | 5.3.1.9 | |
| Enzyme | Isomerase | |
| Storage Conditions |
Storage Temperature: 2-8°C Stability: > 4 years at 4°C |
|
| Application Examples | Applications for the measurement of D-fructose in carbohydrate research and in the food and feeds, fermentation, wine, beverage and dairy industries. | |
| Formulation | In 3.2 M ammonium sulphate | |
| Physical Form | Suspension | |
| CAS Number | 9001-41-6 | |
| Concentration |
E-PGIEC-10KU: ~ 1,000 U/mL. E-PGIEC-50KU: ~ 10,000 U/mL. |
|
| Synonyms | glucose-6-phosphate isomerise; D-glucose-6-phosphate aldose-ketose-isomerase | |
| Source | Escherichia coli | |
| Molecular Weight | 62600 | |
| Expression | Recombinant from Escherichia coli | |
| Specificity | Catalyses the reaction: D-Glucose 6-phosphate = D-fructose 6-phosphate. | |
| Specific Activity |
~ 500 U/mg of protein (25°C, pH 7.6 on fructose 6-phosphate); ~ 650 U/mg of protein (40°C, pH 7.6 on fructose 6-phosphate) |
|
| Unit Definition | One unit of phosphoglucose isomerase activity is the amount of enzyme required to convert one µmole of D-fructose 6-phosphate to D-glucose 6-phosphate per min at pH 7.6. | |
| Temperature Optima (°C) | 40 | |
| pH Optima | 7.5-7.9 | |
| Package Weight | 0.04 lb | |
Documents
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