Phosphatase (Alkaline) (Escherichia coli)

400 Units

Item No.  E-ALPEC

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High purity recombinant Alkaline phosphatase (Escherichia coli) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

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Application Examples Widely used in molecular biology and in enzyme-linked immunosorbent assays (ELISA).
Brand Megazyme
CAS Number 9001-78-9 
Concentration ~ 200 U/mL
EC Number
Enzyme Phosphatase
Expression Recombinant from Escherichia coli
Formulation In 3.2 M ammonium sulphate containing 1 mM MgCl2 and 0.1 mM ZnSO4
Molecular Weight 50503
Package Dimensions 0.70 IN x 0.70 IN x 1.60 IN
Package Weight 0.04 LB
pH Optima 10.0-10.4
Physical Form Suspension
Source Escherichia coli
Specificity Catalyses the reaction: A phosphate monoester + H2O = an alcohol + phosphate
Specific Activity ~ 10 U/mg (37°C, pH 10.4 on p-nitrophenyl phosphate)
Storage Conditions

Storage Temperature: 2-8°C

Stability: Minimum 1 year at 4°C. Check vial for details.

Synonyms alkaline phosphatase; phosphate-monoester phosphohydrolase (alkaline optimum); Orthophosphoric-monoester phosphohydrolase (alkaline optimum)
Temperature Optima (°C) 37
Unit Definition One Unit of alkaline phosphatase is defined as the amount of enzyme required to release one μmole of p-nitrophenol per minute from p-nitrophenyl phosphate (1 mM), in glycine buffer (95.3 mM), pH 10.4 at 37°C.