Pectate Lyase (Aspergillus sp.)

7,000 Units in 50% aqueous glycerol

Item No.  E-PCLYAN

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High purity Pectate lyase (Aspergillus sp.) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

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Specifications
Application Examples Suitable for pectin identification according to USP method [Pectin 9000-69-5]; Pectin Identification Procedure. Applications in carbohydrate and biofuels research.
Brand Megazyme
CAS Number 9015-75-2 
CAZy Family PL1
Concentration ~ 1400 U/mL
EC Number 4.2.2.2
Enzyme Pectate Lyase
Expression Purified from Aspergillus sp.
Formulation In 50% (v/v) glycerol
Molecular Weight 45000
Package Dimensions 0.80 IN x 0.80 IN x 1.90 IN
Package Weight 0.04 LB
pH Optima 8.0-8.4
Physical Form Solution
Source Aspergillus sp.
Specificity Eliminative cleavage of (1,4)-α-D-galacturonan to give oligosaccharides with 4-deoxy-α-D-galact-4-enuronosyl groups at their non-reducing ends
Specific Activity ~ 180 U/mg (40°C, pH 8.0 on polygalacturonic acid)
Storage Conditions

Storage Temperature: Below -10°C

Stability: > 4 years at 4°C or below -10°C

Synonyms pectate lyase; (1→4)-alpha-D-galacturonan lyase
Temperature Optima (°C) 55
Unit Definition One Unit of pectate lyase activity is defined as the amount of enzyme required to release one μmole of galacturonic acid from polygalacturonic acid (2.5 mg/mL) per min in Tris.HCl buffer (50 mM), pH 8.0 at 40°C.

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