myo-Inositol dehydrogenase (Bacillus subtilis)

500 Units

Item No.  E-INDHBS

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High purity recombinant myo-Inositol dehydrogenase (Bacillus subtilis) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

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Specifications
Application Examples Applications for the measurement of myo-inositol acid in the food, fermentation, wine, beverage and dairy industries.
Brand Megazyme
CAS Number 9028-25-5 
Concentration ~ 250 U/mL
EC Number 1.1.1.18
Enzyme Dehydrogenase
Expression Recombinant from Bacillus Subtilis
Formulation In 3.2 M ammonium sulphate
Molecular Weight 39417
Package Dimensions 0.70 IN x 0.70 IN x 1.60 IN
Package Weight 0.04 LB
pH Optima 9.5-9.9
Physical Form Suspension
Source Bacillus subtilis
Specificity Catalyses the reaction: myo-inositol + NAD+ = 2,4,6/3,5-pentahydroxycyclohexanone + NADH + H+
Specific Activity ~ 50 U/mg (25°C, pH 9.6 on myo-inositol)
Storage Conditions

Storage Temperature: 2-8°C

Stability: Minimum 1 year at 4°C. Check vial for details.

Synonyms inositol 2-dehydrogenase; myo-Inositol:NAD+ 2-oxidoreductase
Temperature Optima (°C) 25
Unit Definition One Unit of myo-inositol dehydrogenase activity is defined as the amount of enzyme required to produce one μmole of scyllo-inosose and NADH from myo-inositol (123 mM) and NAD+ per minute in Glycylglycine buffer (492mM), pH 9.6 at 25°C.

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