Lichenase (endo-1,3:1,4-β-D-Glucanase) (Bacillus subtilis)

5,000 Units

Item No.  E-LICHN

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High purity Lichenase (endo-1,3,1,4-β-Glucanase) (Bacillus subtilis) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

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Brand Megazyme
EC Number 
Enzyme β-Glucanase, Lichenase
Storage Conditions

Storage Temperature: 2-8°C

Application Examples Applications in carbohydrate research and in the food and feeds, brewing and biofuels industries.
Formulation In 3.2 M ammonium sulphate
Physical Form Suspension
CAS Number 37288-51-0 
CAZy Family GH16
Concentration ~ 1000 U/mL
Synonyms licheninase; (1→3)-(1→4)-beta-D-glucan 4-glucanohydrolase
Source Bacillus subtilis
Molecular Weight 26750
Expression Purified from Bacillus subtilis
Specificity Hydrolysis of (1,4)-β-D-glucosidic linkages in β-D-glucans containing (1,3)- and (1,4)-bonds.
Specific Activity ~ 230 U/mg (40°C, pH 6.5 on barley β-glucan)
Unit Definition One Unit of lichenase activity is defined as the amount of enzyme required to release one μmole of glucose reducing-sugar equivalents per minute from barley β-glucan (10 mg/mL) in sodium phosphate buffer (100mM), pH 6.5 at 40°C.
Temperature Optima (°C) 60
pH Optima 6.5-6.9
Package Weight 0.04