Isoamylase HP (Glycogen 6-glucanohydrolase)

200 Units

SKU No.  700004224  |  Catalog No.  E-ISAMYHP

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High purity Isoamylase HP (Glycogen 6-glucanohydrolase) for use in research, biochemical enzyme assays and in vitro diagnostic analysis. Suitable for use in ISO Standard 6647-1:2015.

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Specifications
BrandMegazyme
ApprovalsISO Standard 6647-1:2015
EC Number3.2.1.68
EnzymeIsoamylase
Storage Conditions

Storage Temperature: 2-8°C

Stability: > 1 year under recommended storage conditions

Application ExamplesApplications in carbohydrate research and in the food and feeds, and cereals industry.
FormulationIn 3.2 M ammonium sulphate
Physical FormSuspension
CAS Number9067-73-6
CAZy FamilyGH13
Concentration~ 500
Synonymsisoamylase; glycogen 6-alpha-D-glucanohydrolase
SourcePseudomonas sp.
Molecular Weight71500
ExpressionPurified from Pseudomonas sp.
SpecificityHydrolysis of (1,6)-α-D-glucosidic branch linkages in glycogen, amylopectin and their β-limit dextrins.
Specific Activity~ 240 U/mg (40°C, pH 4.0 on oyster glycogen) (equivalent to 16 MU Sigma Units/mg)
Unit DefinitionOne unit of isoamylase activity is the amount of enzyme required to release one μmole of D-glucose reducing sugar equivalent in the presence of oyster glycogen per min at pH 4.0 and 40°C.
Temperature Optima (°C)50
pH Optima4
Package Dimensions0.30 in x 0.30 in x 1.80 in
Package Weight0.03 lb