Isoamylase HP (Glycogen 6-glucanohydrolase)

200 Units


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High purity Isoamylase HP (Glycogen 6-glucanohydrolase) for use in research, biochemical enzyme assays and in vitro diagnostic analysis. Suitable for use in ISO Standard 6647-1:2015.

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Application Examples Applications in carbohydrate research and in the food and feeds, and cereals industry.
Approvals ISO Standard 6647-1:2015
Brand Megazyme
CAS Number 9067-73-6 
CAZy Family GH13
Concentration ~ 500 U/mL
EC Number 
Enzyme Isoamylase
Expression Purified from Pseudomonas sp.
Formulation In 3.2 M ammonium sulphate
Molecular Weight 71500
Package Dimensions 0.30 IN x 0.30 IN x 1.80 IN
Package Weight 0.03 LB
pH Optima 4.0-4.4
Physical Form Suspension
Source Pseudomonas sp.
Specificity Hydrolysis of (1,6)-α-D-glucosidic branch linkages in glycogen, amylopectin and their β-limit dextrins.
Specific Activity ~ 240 U/mg (40°C, pH 4.0 on oyster glycogen) (equivalent to 16 MU Sigma Units/mg)
Storage Conditions

Storage Temperature: 2-8°C

Stability: > 4 years at 4°C

Synonyms isoamylase; glycogen 6-alpha-D-glucanohydrolase
Temperature Optima (°C) 50
Unit Definition One unit of isoamylase activity is the amount of enzyme required to release one μmole of D-glucose reducing sugar equivalent in the presence of oyster glycogen per min at pH 4.0 and 40°C.