Isoamylase (Glycogen 6-glucanohydrolase) 

600 Units

Item No.  E-ISAMY

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Pure Isoamylase (Glycogen 6-glucanohydrolase) for use in biochemical enzyme assays and in vitro diagnostic analysis. Isoamylase, Fructanase (E-FRMXPD) and Amyloglucosidase (E-AMGDF) are used in the enzyme hydrolysis step of two validated methods for the determination of polydextrose (a low molar mass dietary fiber) in foods: AOAC method 2000.11 and Chinese GB Standard 5009.245-2016.

Please refer to E-ISAMYHP for high purity enzyme suitable for use in starch structural research.

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Specifications
Application Examples Applications in carbohydrate research and in the food and feeds, and cereals industry.
Approvals AOAC Method 2000.11 and GB Standard 5009.245-2016
Brand Megazyme
CAS Number 9067-73-6 
CAZy Family GH13
Concentration ~ 200 U/mL
EC Number 3.2.1.68 
Enzyme Isoamylase
Expression Purified from Pseudomonas sp.
Formulation In 3.2 M ammonium sulphate
Molecular Weight 71500
Package Dimensions 0.70 IN x 0.70 IN x 1.60 IN
Package Weight 0.03 LB
pH Optima 4.0-4.4
Physical Form Suspension
Source Pseudomonas sp.
Specificity Hydrolysis of (1,6)-α-D-glucosidic branch linkages in glycogen, amylopectin and their β-limit dextrins.
Specific Activity ~ 180 U/mg (40°C, pH 4.0 on oyster glycogen) (equivalent to 16 MU Sigma Units/mg).
Storage Conditions

Storage Temperature: 2-8°C

Stability: > 4 years at 4°C

Synonyms isoamylase; glycogen 6-alpha-D-glucanohydrolase
Temperature Optima (°C) 50
Unit Definition One unit of isoamylase activity is the amount of enzyme required to release one μmole of D-glucose reducing sugar equivalent in the presence of oyster glycogen per min at pH 4.0 and 40°C.

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