Isoamylase (Glycogen 6-glucanohydrolase) (Flavobacterium odoratum)

Item No.  E-ISAMYFO-800U

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Pure Isoamylase (Glycogen 6-glucanohydrolase) (Flavobacterium odoratum) for use in biochemical enzyme assays and in vitro diagnostic analysis. Isoamylase, Fructanase (E-FRMXPD) and Amyloglucosidase (E-AMGDF) are used in the enzyme hydrolysis step of two validated methods for the determination of polydextrose (a low molar mass dietary fiber) in foods: AOAC method 2000.11 and Chinese GB Standard 5009.245-2016.

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Specifications
Brand Megazyme
Approvals AOAC Method 2000.11 and GB Standard 5009.245-2016
EC Number 3.2.1.68
Enzyme Isoamylase
Storage Conditions

Storage Temperature: 4°C

Stability: > 4 years at 4°C

Application Examples Applications in carbohydrate research and in the food and feeds, and cereals industry.
Formulation In 3.2 M ammonium sulphate
Physical Form Suspension
CAS Number 9067-73-6
CAZy Family GH13
Concentration ~ 200 U/mL
Synonyms isoamylase; glycogen 6-alpha-D-glucanohydrolase
Source Flavobacterium odoratum
Molecular Weight 83000
Expression Purified from Flavobacterium odoratum
Specificity Hydrolysis of (1,6)-α-D-glucosidic branch linkages in glycogen, amylopectin and their β-limit dextrins.
Specific Activity ~ 140 U/mg (40°C, pH 6.0 with 1 mM CaCl2 on oyster glycogen)
Unit Definition One Unit of isoamylase activity is defined as the amount of enzyme required to release one µmole of glucose reducing sugar equivalents per minute from oyster glycogen (10 mg/mL) in MES buffer (100 mM, pH 6.0) plus 1 mM CaCl2 at 40°C.
Temperature Optima (°C) 50
pH Optima 6.0-6.4
Package Weight 0.03 lb

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