Isoamylase (Glycogen 6-glucanohydrolase) (Flavobacterium odoratum)
Item No. E-ISAMYFO-800U
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| Specifications | ||
|---|---|---|
| Brand | Megazyme | |
| Approvals | AOAC Method 2000.11 and GB Standard 5009.245-2016 | |
| EC Number | 3.2.1.68 | |
| Enzyme | Isoamylase | |
| Storage Conditions |
Storage Temperature: 4°C |
|
| Application Examples | Applications in carbohydrate research and in the food and feeds, and cereals industry. | |
| Formulation | In 3.2 M ammonium sulphate | |
| Physical Form | Suspension | |
| CAS Number | 9067-73-6 | |
| CAZy Family | GH13 | |
| Concentration | ~ 200 U/mL | |
| Synonyms | isoamylase; glycogen 6-alpha-D-glucanohydrolase | |
| Source | Flavobacterium odoratum | |
| Molecular Weight | 83000 | |
| Expression | Purified from Flavobacterium odoratum | |
| Specificity | Hydrolysis of (1,6)-α-D-glucosidic branch linkages in glycogen, amylopectin and their β-limit dextrins. | |
| Specific Activity | ~ 140 U/mg (40°C, pH 6.0 with 1 mM CaCl2 on oyster glycogen) | |
| Unit Definition | One Unit of isoamylase activity is defined as the amount of enzyme required to release one µmole of glucose reducing sugar equivalents per minute from oyster glycogen (10 mg/mL) in MES buffer (100 mM, pH 6.0) plus 1 mM CaCl2 at 40°C. | |
| Temperature Optima (°C) | 50 | |
| pH Optima | 6.0-6.4 | |
| Package Weight | 0.03 | |