Isoamylase (Glycogen 6-glucanohydrolase) (Flavobacterium odoratum)

800 Units

SKU No.  700004223  |  Catalog No.  E-ISAMYFO-800U

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Pure Isoamylase (Glycogen 6-glucanohydrolase) (Flavobacterium odoratum) for use in biochemical enzyme assays and in vitro diagnostic analysis. Isoamylase, Fructanase (E-FRMXPD) and Amyloglucosidase (E-AMGDF) are used in the enzyme hydrolysis step of two validated methods for the determination of polydextrose (a low molar mass dietary fiber) in foods: AOAC method 2000.11 and Chinese GB Standard 5009.245-2016.

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Specifications
BrandMegazyme
ApprovalsAOAC Method 2000.11 and GB Standard 5009.245-2016
EC Number3.2.1.68
EnzymeIsoamylase
Storage Conditions

Storage Temperature: 4°C

Stability: > 1 year under recommended storage conditions

Application ExamplesApplications in carbohydrate research and in the food and feeds, and cereals industry.
FormulationIn 3.2 M ammonium sulphate
Physical FormSuspension
CAS Number9067-73-6
CAZy FamilyGH13
Concentration~ 200
Synonymsisoamylase; glycogen 6-alpha-D-glucanohydrolase
SourceFlavobacterium odoratum
Molecular Weight83000
ExpressionPurified from Flavobacterium odoratum
SpecificityHydrolysis of (1,6)-α-D-glucosidic branch linkages in glycogen, amylopectin and their β-limit dextrins.
Specific Activity~ 140 U/mg (40°C, pH 6.0 with 1 mM CaCl2 on oyster glycogen)
Unit DefinitionOne Unit of isoamylase activity is defined as the amount of enzyme required to release one µmole of glucose reducing sugar equivalents per minute from oyster glycogen (10 mg/mL) in MES buffer (100 mM, pH 6.0) plus 1 mM CaCl2 at 40°C.
Temperature Optima (°C)50
pH Optima6
Package Dimensions0.80 in x 0.80 in x 1.90 in
Package Weight0.03 lb

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