Hexokinase/Glucose-6-phosphate dehydrogenase

10 mL Hexokinase (420 U/mL) + G6P-DH (210 U/mL)

Item No.  E-HKGDH

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High purity Hexokinase (yeast) / Glucose-6-phosphate dehydrogenase (G6P-DH) (Leuconostoc mesenteroides) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

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Megazyme_Scheme-E-HKGDH.png
Specifications
Application Examples Applications for the measurement of glucose and other hexoses in carbohydrate research and in the food and feeds, fermentation, wine, beverage and dairy industries.
Brand Megazyme
CAS Number Hexokinase: 9001-51-8
G6P-DH: 9001-40-5
Concentration Hexokinase: ~ 420 U/mL
G6P-DH: ~ 210 U/mL
EC Number Hexokinase: 2.7.1.1
G6P-DH: 1.1.1.49
Enzyme Kinase
Expression Hexokinase: Recombinant from yeast
G6P-DH: Recombinant from Leuconostoc mesenteroides
Formulation In 3.2 M ammonium sulphate
Package Dimensions 0.80 IN x 0.80 IN x 1.90 IN
Package Weight 0.06 LB
pH Optima 7.0-7.4
Physical Form Suspension
Source Yeast
Specificity Hexokinase: Catalyses the reaction: ATP + D-hexose = ADP + D-hexose 6-phosphate Phopshorylates D-glucose, D-mannose, D-fructose, sorbitol and D-glucosamine.
Glucose-6-phosphate dehydrogenase: Catalyses the reaction: D-glucose 6-phosphate + NADP+ = 6-phospho-D-glucono-1,5-lactone + NADPH + H+
Storage Conditions

Storage Temperature: 2-8°C

Stability: > 4 years at 4°C

Synonyms Hexokinase: Hexokinase; ATP:D-hexose 6-phosphotransferase
G6P-DH: glucose-6-phosphate dehydrogenase (NADP+); D-glucose-6-phosphate:NADP+ 1-oxidoreductase
Temperature Optima (°C) 40
Unit Definition Hexokinase: One Unit of hexokinase activity is defined as the amount of enzyme required to produce one µmole of NADH from NAD+ in the presence of D-glucose and glucose-6-phosphate dehydrogenase at pH 7.5 and 25°C.
G6P-DH: One Unit of Glucose-6-phosphate dehydrogenase activity is the amount of enzyme required to convert one μmole of glucose-6-phosphate to 6-phosphogluconate per minute, in the presence of NADP+ at pH 7.8 and 25°C.

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