Hexokinase/Glucose-6-phosphate dehydrogenase

10 ml Hexokinase (420 U/ml) + G6P-DH (210 U/ml)

Item No.  E-HKGDH

This item has shipping restrictions.
$207.00 each
In stock
Out of stock
Expect availability in 2-4 weeks
Qty:
Minimum quantity required: 1
This item must be ordered in multiples of one case. Quantity per case: 1

High purity Hexokinase (yeast) / Glucose-6-phosphate dehydrogenase (G6P-DH) (Leuconostoc mesenteroides) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

View Documents

Megazyme_Scheme-E-HKGDH.png
Specifications
Brand Megazyme
EC Number Hexokinase: 2.7.1.1
G6P-DH: 1.1.1.49
Enzyme Kinase
Storage Conditions

Storage Temperature: 2-8°C

Stability: > 4 years at 4°C

Application Examples Applications for the measurement of glucose and other hexoses in carbohydrate research and in the food and feeds, fermentation, wine, beverage and dairy industries.
Formulation In 3.2 M ammonium sulphate
Physical Form Suspension
CAS Number Hexokinase: 9001-51-8
G6P-DH: 9001-40-5
Concentration Hexokinase: ~ 420 U/mL
G6P-DH: ~ 210 U/mL
Synonyms Hexokinase: Hexokinase; ATP:D-hexose 6-phosphotransferase
G6P-DH: glucose-6-phosphate dehydrogenase (NADP+); D-glucose-6-phosphate:NADP+ 1-oxidoreductase
Source Yeast
Expression Hexokinase: Recombinant from yeast
G6P-DH: Recombinant from Leuconostoc mesenteroides
Specificity Hexokinase: Catalyses the reaction: ATP + D-hexose = ADP + D-hexose 6-phosphate Phopshorylates D-glucose, D-mannose, D-fructose, sorbitol and D-glucosamine.
Glucose-6-phosphate dehydrogenase: Catalyses the reaction: D-glucose 6-phosphate + NADP+ = 6-phospho-D-glucono-1,5-lactone + NADPH + H+
Unit Definition Hexokinase: One Unit of hexokinase activity is defined as the amount of enzyme required to produce one µmole of NADH from NAD+ in the presence of D-glucose and glucose-6-phosphate dehydrogenase at pH 7.5 and 25°C.
G6P-DH: One Unit of Glucose-6-phosphate dehydrogenase activity is the amount of enzyme required to convert one μmole of glucose-6-phosphate to 6-phosphogluconate per minute, in the presence of NADP+ at pH 7.8 and 25°C.
Temperature Optima (°C) 40
pH Optima 7.0-7.4
Package Weight 0.06 lb

Support