Hexokinase/Glucose-6-phosphate dehydrogenase
10 ml Hexokinase (420 U/ml) + G6P-DH (210 U/ml)
Item No. 700004221 | Catalog No. E-HKGDH
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High purity Hexokinase (yeast) / Glucose-6-phosphate dehydrogenase (G6P-DH) (Leuconostoc mesenteroides) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
| Specifications | ||
|---|---|---|
| Brand | Megazyme | |
| EC Number |
Hexokinase: 2.7.1.1 G6P-DH: 1.1.1.49 |
|
| Enzyme | Kinase | |
| Storage Conditions |
Storage Temperature: 2-8°C Stability: > 4 years at 4°C |
|
| Application Examples | Applications for the measurement of glucose and other hexoses in carbohydrate research and in the food and feeds, fermentation, wine, beverage and dairy industries. | |
| Formulation | In 3.2 M ammonium sulphate | |
| Physical Form | Suspension | |
| CAS Number |
Hexokinase: 9001-51-8 G6P-DH: 9001-40-5 |
|
| Concentration |
Hexokinase: ~ 420 U/mL G6P-DH: ~ 210 U/mL |
|
| Synonyms |
Hexokinase: Hexokinase; ATP:D-hexose 6-phosphotransferase G6P-DH: glucose-6-phosphate dehydrogenase (NADP+); D-glucose-6-phosphate:NADP+ 1-oxidoreductase |
|
| Source | Yeast | |
| Expression |
Hexokinase: Recombinant from yeast G6P-DH: Recombinant from Leuconostoc mesenteroides |
|
| Specificity |
Hexokinase: Catalyses the reaction: ATP + D-hexose = ADP + D-hexose 6-phosphate Phopshorylates D-glucose, D-mannose, D-fructose, sorbitol and D-glucosamine. Glucose-6-phosphate dehydrogenase: Catalyses the reaction: D-glucose 6-phosphate + NADP+ = 6-phospho-D-glucono-1,5-lactone + NADPH + H+ |
|
| Unit Definition |
Hexokinase: One Unit of hexokinase activity is defined as the amount of enzyme required to produce one µmole of NADH from NAD+ in the presence of D-glucose and glucose-6-phosphate dehydrogenase at pH 7.5 and 25°C. G6P-DH: One Unit of Glucose-6-phosphate dehydrogenase activity is the amount of enzyme required to convert one μmole of glucose-6-phosphate to 6-phosphogluconate per minute, in the presence of NADP+ at pH 7.8 and 25°C. |
|
| Temperature Optima (°C) | 40 | |
| pH Optima | 7.4 | |
| Package Dimensions | 0.80 in x 0.80 in x 1.90 in | |
| Package Weight | 0.06 lb | |
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