Hexokinase/Glucose-6-phosphate dehydrogenase

10 ml Hexokinase (420 U/ml) + G6P-DH (210 U/ml)

Item No.  E-HKGDH

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High purity Hexokinase (yeast) / Glucose-6-phosphate dehydrogenase (G6P-DH) (Leuconostoc mesenteroides) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

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Brand Megazyme
EC Number Hexokinase:
Enzyme Kinase
Storage Conditions

Storage Temperature: 2-8°C

Stability: > 4 years at 4°C

Application Examples Applications for the measurement of glucose and other hexoses in carbohydrate research and in the food and feeds, fermentation, wine, beverage and dairy industries.
Formulation In 3.2 M ammonium sulphate
Physical Form Suspension
CAS Number Hexokinase: 9001-51-8
G6P-DH: 9001-40-5
Concentration Hexokinase: ~ 420 U/mL
G6P-DH: ~ 210 U/mL
Synonyms Hexokinase: Hexokinase; ATP:D-hexose 6-phosphotransferase
G6P-DH: glucose-6-phosphate dehydrogenase (NADP+); D-glucose-6-phosphate:NADP+ 1-oxidoreductase
Source Yeast
Expression Hexokinase: Recombinant from yeast
G6P-DH: Recombinant from Leuconostoc mesenteroides
Specificity Hexokinase: Catalyses the reaction: ATP + D-hexose = ADP + D-hexose 6-phosphate Phopshorylates D-glucose, D-mannose, D-fructose, sorbitol and D-glucosamine.
Glucose-6-phosphate dehydrogenase: Catalyses the reaction: D-glucose 6-phosphate + NADP+ = 6-phospho-D-glucono-1,5-lactone + NADPH + H+
Unit Definition Hexokinase: One Unit of hexokinase activity is defined as the amount of enzyme required to produce one µmole of NADH from NAD+ in the presence of D-glucose and glucose-6-phosphate dehydrogenase at pH 7.5 and 25°C.
G6P-DH: One Unit of Glucose-6-phosphate dehydrogenase activity is the amount of enzyme required to convert one μmole of glucose-6-phosphate to 6-phosphogluconate per minute, in the presence of NADP+ at pH 7.8 and 25°C.
Temperature Optima (°C) 40
pH Optima 7.0-7.4
Package Weight 0.06