Glucose-6-phosphate dehydrogenase (Escherichia coli)

5,000 Units

Item No.  E-GPDHEC

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High purity recombinant Glucose-6-phosphate dehydrogenase (Escherichia coli) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

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Specifications
Application Examples Applications for the measurement of glucose and other hexoses in carbohydrate research and in the food and feeds, fermentation, wine, beverage and dairy industries.
Brand Megazyme
CAS Number 9001-40-5
Concentration ~ 1250 U/mL
EC Number 1.1.1.49
Enzyme Dehydrogenase
Expression Recombinant from Escherichia coli
Formulation In 3.2 M ammonium sulphate
Molecular Weight 56770
Package Dimensions 0.70 IN x 0.70 IN x 1.60 IN
Package Weight 0.04 LB
pH Optima 7.5-7.9
Physical Form Suspension
Source Escherichia coli
Specificity Catalyses the reaction: D-glucose 6-phosphate + NADP+ = 6-phospho-D-glucono-1,5-lactone + NADPH + H+
Specific Activity ~ 160 U/mg (25°C, pH 7.6 on D-glucose-6-phosphate)
Storage Conditions

Storage Temperature: 2-8°C

Stability: Minimum 1 year at 4oC. Check vial for details.

Synonyms glucose-6-phosphate dehydrogenase (NADP+); D-glucose-6-phosphate:NADP+ 1-oxidoreductase
Temperature Optima (°C) 25
Unit Definition One Unit of Glucose-6-phosphate dehydrogenase activity is defined as the amount of enzyme required to convert one μmole of glucose 6-phosphate (5.0 mM) to 6-phosphogluconate per minute in the presence of NADP+ in imidazole buffer (168 mM), pH 7.6 at 25°C.

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