Galactose dehydrogenase - Galactose mutarotase
1 ml; galactose dehydrogenase (200 U/ml) / galactose mutarotase (4 mg/ml)
Item No. E-GALMUT
High purity Galactose dehydrogenase (Escherichia coli) / Galactose mutarotase (Escherichia coli) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
Can be employed for the rapid determination of D-galactose or L-arabinose. See Lactose/D-Galactose (Rapid) Assay Kit (K-LACGAR) for full details.
For use under Patent No. US 7,785,771 B2 and EP1 828 407 (GB, FR, IE, DE)
| Specifications | ||
|---|---|---|
| Brand | Megazyme | |
| EC Number |
Galactose dehydrogenase: 1.1.1.48 Galactose mutarotase: 5.1.3.3 |
|
| Enzyme | Dehydrogenase | |
| Storage Conditions |
Storage Temperature: 2-8°C Stability: Minimum 1 year at 4°C. Check vial for details. |
|
| Application Examples | Used for the rapid measurement of D-galactose or L-arabinose in the L-Arabinose/D-Galactose Assay Kit (K-ARGA). Also used in the measurement of lactose via d-galactose in the Lactose/D-Galactose (Rapid) Assay Kit (K-LACGAR). | |
| Formulation | In 3.2 M ammonium sulphate | |
| Physical Form | Suspension | |
| CAS Number |
Galactose dehydrogenase: 9028-54-0 Galactose mutarotase: 9031-76-9 |
|
| Synonyms |
Galactose dehydrogenase: D-galactose 1-dehydrogenase; D-galactose:NAD+ 1-oxidoreductase Galactose mutarotase: aldose 1-epimerase; aldose 1-epimerase |
|
| Source | Escherichia coli | |
| Expression | Recombinant from Escherichia coli | |
| Specificity |
Interconversion of the α- and β-anomeric forms of D-galactose is catalysed by β-galactose mutarotase (GMR) (1). (1) α-D-Galactose ↔ β-D-Galactose. The β-D-Galactose is oxidised by NAD+ to D-galactonic acid in the presence of β-galactose dehydrogenase (GALDH) at pH 7.5 (2). (2) β-D-Galactose + NAD+ → D-galactonic acid + NADH + H+ |
|
| Specific Activity |
Galactose dehydrogenase: ~ 200 U/mL at pH 8.6 and 25°C Galactose mutarotase: 4.1 mg/mL |
|
| Unit Definition | One Unit of galactose dehydrogenase is defined as the amount of enzyme required to produce one μmole of NADH from NAD+ per minute at pH 8.6, at 25°C. | |
| Temperature Optima (°C) | 25 | |
| pH Optima | 8.5-8.9 | |
| Package Weight | 0.04 lb | |
Documents
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