Galactose dehydrogenase - Galactose mutarotase 

1 ml; galactose dehydrogenase (200 U/ml) / galactose mutarotase (4 mg/ml)

Item No.  E-GALMUT

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High purity Galactose dehydrogenase (Escherichia coli) / Galactose mutarotase (Escherichia coli) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

Can be employed for the rapid determination of D-galactose or L-arabinose. See Lactose/D-Galactose (Rapid) Assay Kit (K-LACGAR) for full details.

For use under Patent No. US 7,785,771 B2 and EP1 828 407 (GB, FR, IE, DE)

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Megazyme_Scheme-E-GALMUT.png
Specifications
Brand Megazyme
EC Number Galactose dehydrogenase: 1.1.1.48
Galactose mutarotase: 5.1.3.3
Enzyme Dehydrogenase
Storage Conditions

Storage Temperature: 2-8°C

Stability: Minimum 1 year at 4°C. Check vial for details.

Application Examples Used for the rapid measurement of D-galactose or L-arabinose in the L-Arabinose/D-Galactose Assay Kit (K-ARGA). Also used in the measurement of lactose via d-galactose in the Lactose/D-Galactose (Rapid) Assay Kit (K-LACGAR).
Formulation In 3.2 M ammonium sulphate
Physical Form Suspension
CAS Number Galactose dehydrogenase: 9028-54-0
Galactose mutarotase: 9031-76-9
Synonyms Galactose dehydrogenase: D-galactose 1-dehydrogenase; D-galactose:NAD+ 1-oxidoreductase
Galactose mutarotase: aldose 1-epimerase; aldose 1-epimerase
Source Escherichia coli
Expression Recombinant from Escherichia coli
Specificity

Interconversion of the α- and β-anomeric forms of D-galactose is catalysed by β-galactose mutarotase (GMR) (1).

(1) α-D-Galactose ↔ β-D-Galactose.

The β-D-Galactose is oxidised by NAD+ to D-galactonic acid in the presence of β-galactose dehydrogenase (GALDH) at pH 7.5 (2).

(2) β-D-Galactose + NAD+ → D-galactonic acid + NADH + H+

Specific Activity Galactose dehydrogenase: ~ 200 U/mL at pH 8.6 and 25°C
Galactose mutarotase: 4.1 mg/mL
Unit Definition One Unit of galactose dehydrogenase is defined as the amount of enzyme required to produce one μmole of NADH from NAD+ per minute at pH 8.6, at 25°C.
Temperature Optima (°C) 25
pH Optima 8.5-8.9
Package Weight 0.04 lb

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