Formate dehydrogenase (Candida boidinii)

300 Units at 25°C; ~ 600 Units at 37°C

Item No.  E-FDHCB

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High purity recombinant Formate dehydrogenase (Candida boidinii) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

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Specifications
Application Examples Applications for the measurement of formate in the food, fermentation, wine, beverage and dairy industries.
Brand Megazyme
CAS Number 9028-85-7
Concentration ~ 75 U/mL
EC Number 1.2.1.2 (transferred to EC 1.17.1.9)
Enzyme Dehydrogenase
Expression Recombinant from Candida boidinii
Formulation In 3.2 M ammonium sulphate
Molecular Weight 41331
Package Dimensions 0.70 IN x 0.70 IN x 1.60 IN
Package Weight 0.03 LB
pH Optima 7.5-7.9
Physical Form Suspension
Source Candida boidinii
Specificity Catalyses the reaction: Formate + NAD+ = CO2 + NADH
Specific Activity ~ 2 U/mg (25°C, pH 7.6 on formic acid)
Storage Conditions

Storage Temperature: 2-8°C

Stability: Minimum 1 year at 4°C. Check vial for details.

Synonyms formate dehydrogenase; formate:NAD+ oxidoreductase
Temperature Optima (°C) 37
Unit Definition One Unit of formate dehydrogenase is defined as the amount of enzyme required to convert one μmole of formic acid to NADH + CO2 per minute in the presence of NAD+ in potassium phosphate buffer (41 mM), pH 7.6 at 25°C.

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