Formate dehydrogenase (Candida boidinii)
300 Units at 25°C; ~ 600 Units at 37°C
Item No. E-FDHCB
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High purity recombinant Formate dehydrogenase (Candida boidinii) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
| Specifications | ||
|---|---|---|
| Brand | Megazyme | |
| EC Number | 1.2.1.2 (transferred to EC 1.17.1.9) | |
| Enzyme | Dehydrogenase | |
| Storage Conditions |
Storage Temperature: 2-8°C Stability: Minimum 1 year at 4°C. Check vial for details. |
|
| Application Examples | Applications for the measurement of formate in the food, fermentation, wine, beverage and dairy industries. | |
| Formulation | In 3.2 M ammonium sulphate | |
| Physical Form | Suspension | |
| CAS Number | 9028-85-7 | |
| Concentration | ~ 75 U/mL | |
| Synonyms | formate dehydrogenase; formate:NAD+ oxidoreductase | |
| Source | Candida boidinii | |
| Molecular Weight | 41331 | |
| Expression | Recombinant from Candida boidinii | |
| Specificity | Catalyses the reaction: Formate + NAD+ = CO2 + NADH | |
| Specific Activity | ~ 2 U/mg (25°C, pH 7.6 on formic acid) | |
| Unit Definition | One Unit of formate dehydrogenase is defined as the amount of enzyme required to convert one μmole of formic acid to NADH + CO2 per minute in the presence of NAD+ in potassium phosphate buffer (41 mM), pH 7.6 at 25°C. | |
| Temperature Optima (°C) | 37 | |
| pH Optima | 7.5-7.9 | |
| Package Weight | 0.03 | |