exo-α-Sialidase (Clostridium perfringens)
50 Units
Item No. E-SIALCP
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High purity recombinant exo-α-sialidase (Clostridium perfringens) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
| Specifications | ||
|---|---|---|
| Brand | Megazyme | |
| EC Number | 3.2.1.18 | |
| Enzyme | α-Sialidase | |
| Storage Conditions |
Storage Temperature: 2-8°C |
|
| Application Examples | Applications in clinical chemistry, glycobiology and carbohydrate research. | |
| Formulation | In 20 mM tris buffer containing 50 mM NaCl2 and 5 mM EDTA. | |
| Physical Form | Suspension | |
| CAS Number | 9001-67-6 | |
| CAZy Family | GH33 | |
| Concentration | ~ 500 U/mL | |
| Synonyms | exo-alpha-sialidase; acetylneuraminyl hydrolase | |
| Source | Clostridium perfringens | |
| Molecular Weight | 43600 | |
| Expression | Recombinant from Clostridium perfringens | |
| Specificity | Hydrolysis of unbranched, non-reducing terminal α-2,3-linked, α-2,6-linked >> α-2,8-linked N-acetylneuraminic acid (NANA; Neu5Ac) residues from glycoproteins and oligosaccharides of glycoconjugates. | |
| Specific Activity | ~ 300 U/mg (37°C, pH 7.0 on pNP-α-D-N-acetylneuraminic acid) | |
| Unit Definition | One Unit of sialidase activity is defined as the amount of enzyme required to release one µmole of p-nitrophenol per minute from pNP-α-D-N-acetylneuraminic acid (1 mM) in sodium phosphate buffer (100 mM), pH 7.0 at 37°C. | |
| Temperature Optima (°C) | 37 | |
| pH Optima | 7.0-7.4 | |
| Package Weight | 0.01 | |