exo-α-Sialidase (Clostridium perfringens)

50 Units

Item No.  E-SIALCP

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High purity recombinant exo-α-sialidase (Clostridium perfringens) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

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Specifications
Brand Megazyme
EC Number 3.2.1.18
Enzyme α-Sialidase
Storage Conditions

Storage Temperature: 2-8°C

Stability: Minimum 1 year at 4°C. Check vial for details.

Application Examples Applications in clinical chemistry, glycobiology and carbohydrate research.
Formulation In 20 mM tris buffer containing 50 mM NaCl2 and 5 mM EDTA.
Physical Form Suspension
CAS Number 9001-67-6
CAZy Family GH33
Concentration ~ 500 U/mL
Synonyms exo-alpha-sialidase; acetylneuraminyl hydrolase
Source Clostridium perfringens
Molecular Weight 43600
Expression Recombinant from Clostridium perfringens
Specificity Hydrolysis of unbranched, non-reducing terminal α-2,3-linked, α-2,6-linked >> α-2,8-linked N-acetylneuraminic acid (NANA; Neu5Ac) residues from glycoproteins and oligosaccharides of glycoconjugates.
Specific Activity ~ 300 U/mg (37°C, pH 7.0 on pNP-α-D-N-acetylneuraminic acid)
Unit Definition One Unit of sialidase activity is defined as the amount of enzyme required to release one µmole of p-nitrophenol per minute from pNP-α-D-N-acetylneuraminic acid (1 mM) in sodium phosphate buffer (100 mM), pH 7.0 at 37°C.
Temperature Optima (°C) 37
pH Optima 7.0-7.4
Package Dimensions 0.30 in x 0.30 in x 1.80 in
Package Weight 0.01 lb

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