exo-1,3-β-D-Glucanase + β-Glucosidase

300 Units exo-1,3-β-glucanase / 60 Units β-glucosidase

Item No.  E-EXBGOS

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High purity exo-1,3-β-D-Glucanase (Trichoderma sp.) + β-Glucosidase (Aspergillus niger) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

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Specifications
Application Examples For use in the determination of (1,3)(1,4) β-glucan.
Brand Megazyme
CAS Number exo-1,3-β-glucanase: 9073-49-8
β-glucosidase: 9001-22-3
CAZy Family GH55, GH3
Concentration exo-1,3-β-Glucanase (100 U/mL) / β-Glucosidase (20 U/mL)
EC Number exo-1,3-β-glucanase: 3.2.1.58
β-glucosidase: 3.2.1.21
Enzyme exo-1,3-β-Glucanase, β-Glucosidase
Expression exo-1,3-β-D-Glucanase: Recombinant from Trichoderma sp.
β-Glucosidase: Purified from Aspergillus niger
Formulation In 3.2 M ammonium sulphate
Package Dimensions 0.80 IN x 0.80 IN x 1.90 IN
Package Weight 0.03 LB
pH Optima 4.0-4.4
Physical Form Suspension
Source exo-1,3-β-Glucanase: Trichoderma virens, β-Glucosidase: Aspergillus niger
Specificity exo-1,3-β-glucanase: Successive hydrolysis of β-D-glucose units from the non-reducing ends of (1,3)-β-D-glucans, releasing α-glucose.
β-glucosidase: Hydrolysis of terminal, non-reducing β-D-glucosyl residues with release of β-D-glucose.
Storage Conditions

Storage Temperature: 2-8°C

Stability: Minimum 1 year at 4°C. Check vial for details.

Synonyms exo-1,3-β-Glucanase: glucan 1,3-beta-glucosidase;
3-beta-D-glucan glucohydrolase, β-Glucosidase: beta-glucosidase
Temperature Optima (°C) 40
Unit Definition exo-1,3-β-D-Glucanase: One Unit of exo-1,3-β-glucanase activity is defined as the amount of enzyme required to release one μmole of glucose reducing-sugar equivalents per minute from laminarin (10 mg/mL) in sodium acetate buffer (100 mM), pH 4.0 at 40°C.
β-Glucosidase: One Unit of β-glucosidase activity is defined as the amount of enzyme required to release one μmole of p-nitrophenol per minute from 4-nitrophenyl-β-D-glucpyranoside in sodium acetate buffer (100 mM), pH 4.0 at 40°C.

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