endo-Polygalacturonanase (Pectobacterium carotovorum)

5,000 Units

SKU No.  700004232  |  Catalog No.  E-PGALPC

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Minimum quantity required: 1
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High purity endo-Polygalacturonase (Pectobacterium carotovorum) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

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Specifications
BrandMegazyme
EC Number3.2.1.15
Enzymeendo-Polygalacturonase
Storage Conditions

Storage Temperature: 2-8°C

Stability: > 1 year under recommended storage conditions

Application ExamplesApplications in carbohydrate research and in the food industry.
FormulationIn 3.2 M ammonium sulphate
Physical FormSuspension
CAS Number9032-75-1
CAZy FamilyGH28
Concentration~ 14000
Synonymspolygalacturonase; (1→4)-alpha-D-galacturonan glycanohydrolase
SourcePectobacterium carotovorum
Molecular Weight39818
ExpressionRecombinant from Pectobacterium carotovorum
SpecificityRandom hydrolysis of α-1,4-D-galactosiduronic linkages in pectate and polygalacturonans.
Specific Activity~ 400 U/mg (40°C, pH 5.5 on polygalacturonic acid)
Unit DefinitionOne Unit of endo-polygalacturonase activity is defined as the amount of enzyme required to release one μmole of galacturonic acid from polygalacturonic acid (5 mg/mL) per min in sodium acetate buffer (100 mM), pH 5.5 at 40°C.
Temperature Optima (°C)50
pH Optima6
Package Dimensions0.70 in x 0.70 in x 1.60 in
Package Weight0.04 lb