endo-Polygalacturonanase (Pectobacterium carotovorum)

5,000 Units

Item No.  E-PGALPC

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High purity endo-Polygalacturonase (Pectobacterium carotovorum) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

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Specifications
Brand Megazyme
EC Number 3.2.1.15
Enzyme endo-Polygalacturonase
Storage Conditions

Storage Temperature: 2-8°C

Stability: > 4 years at 4°C

Application Examples Applications in carbohydrate research and in the food industry.
Formulation In 3.2 M ammonium sulphate
Physical Form Suspension
CAS Number 9032-75-1
CAZy Family GH28
Concentration ~ 14000 U/mL
Synonyms polygalacturonase; (1→4)-alpha-D-galacturonan glycanohydrolase
Source Pectobacterium carotovorum
Molecular Weight 39818
Expression Recombinant from Pectobacterium carotovorum
Specificity Random hydrolysis of α-1,4-D-galactosiduronic linkages in pectate and polygalacturonans.
Specific Activity ~ 250 U/mg (40°C, pH 5.5 polygalacturonic acid)
Unit Definition One Unit of endo-polygalacturonase activity is defined as the amount of enzyme required to release one μmole of galacturonic acid from polygalacturonic acid (5 mg/mL) per min in sodium acetate buffer (100 mM), pH 5.5 at 40°C.
Temperature Optima (°C) 50
pH Optima 6.0-6.4
Package Dimensions 0.70 in x 0.70 in x 1.60 in
Package Weight 0.04 lb

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