endo-1,4 β-Mannanase (Cellvibrio japonicus)

10,000 Units

Item No.  E-BMACJ

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High purity recombinant endo-1,4 β-Mannanase (Cellvibrio japonicus) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

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Specifications
Application Examples Applications established in diagnostics and research within the food and feed, carbohydrate, biofuels and paper production industries.
Brand Megazyme
CAS Number 37288-54-3
CAZy Family GH26
Concentration ~ 5000 U/mL
EC Number 3.2.1.78
Enzyme endo-1,4-β-Mannanase
Expression Recombinant from Cellvibrio japonicus
Formulation In 3.2 M ammonium sulphate
Molecular Weight 45200
Package Dimensions 0.70 IN x 0.70 IN x 1.60 IN
Package Weight 0.03 LB
pH Optima 7.0-7.4
Physical Form Suspension
Source Cellvibrio japonicus
Specificity Random hydrolysis of (1,4)-β-D-mannosidic linkages in mannans, galactomannans and glucomannans.
Specific Activity ~ 250 U/mg (40°C, pH 7.0 on carob galactomannan);
~ 420 U/mg (50°C, pH 7.0 on carob galactomannan).
Storage Conditions

Storage Temperature: 2-8°C

Stability: Minimum 1 year at 4°C. Check vial for details.

Synonyms mannan endo-1,4-beta-mannosidase; 4-beta-D-mannan mannanohydrolase
Temperature Optima (°C) 50
Unit Definition One Unit of mannanase activity is defined as the amount of enzyme required to release one µmole of mannose reducing-sugar equivalents per minute from carob galactomannan (5 mg/mL) in sodium phosphate buffer (100 mM), pH 7.0.

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