endo-1,4 β-Mannanase (Aspergillus niger)

600 Units

SKU No.  700004199  |  Catalog No.  E-BMANN

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High purity endo-1,4 β-Mannanase (Aspergillus niger) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

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Specifications
BrandMegazyme
EC Number3.2.1.78
Enzymeendo-1,4-β-Mannanase
Storage Conditions

Storage Temperature: 2-8°C

Stability: > 1 year under recommended storage conditions

Application ExamplesApplications established in diagnostics and research within the food and feed, carbohydrate, biofuels and paper production industries.
FormulationIn 3.2 M ammonium sulphate
Physical FormSuspension
CAS Number37288-54-3
CAZy FamilyGH26
Concentration~ 600
Synonymsmannan endo-1,4-beta-mannosidase; 4-beta-D-mannan mannanohydrolase
SourceAspergillus niger
Molecular Weight48000
ExpressionPurified from Aspergillus niger
SpecificityRandom hydrolysis of (1,4)-β-D-mannosidic linkages in mannans, galactomannans and glucomannans.
Specific Activity~ 50 U/mg (40°C, pH 4.0 on carob galactomannan)
Unit DefinitionOne Unit of mannanase activity is defined as the amount of enzyme required to release one µmole of mannose reducing-sugar equivalents per minute from carob galactomannan (10 mg/mL) in sodium acetate buffer (100 mM), pH 4.0 at 40°C.
Temperature Optima (°C)60
pH Optima3
Package Dimensions0.70 in x 0.70 in x 1.60 in
Package Weight0.02 lb