endo-1,4 β-Mannanase (Aspergillus niger)

600 Units

Item No.  E-BMANN

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High purity endo-1,4 β-Mannanase (Aspergillus niger) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

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Brand Megazyme
EC Number
Enzyme endo-1,4-β-Mannanase
Storage Conditions

Storage Temperature: 2-8°C

Stability: > 4 years at 4°C

Application Examples Applications established in diagnostics and research within the food and feed, carbohydrate, biofuels and paper production industries.
Formulation In 3.2 M ammonium sulphate
Physical Form Suspension
CAS Number 37288-54-3
CAZy Family GH26
Concentration ~ 600 U/mL
Synonyms mannan endo-1,4-beta-mannosidase; 4-beta-D-mannan mannanohydrolase
Source Aspergillus niger
Molecular Weight 48000
Expression Purified from Aspergillus niger
Specificity Random hydrolysis of (1,4)-β-D-mannosidic linkages in mannans, galactomannans and glucomannans.
Specific Activity ~ 50 U/mg (40°C, pH 4.0 on carob galactomannan)
Unit Definition One Unit of mannanase activity is defined as the amount of enzyme required to release one µmole of mannose reducing-sugar equivalents per minute from carob galactomannan (10 mg/mL) in sodium acetate buffer (100 mM), pH 4.0 at 40°C.
Temperature Optima (°C) 60
pH Optima 3.0-3.4
Package Weight 0.02