Diaphorase (Escherichia coli)

1,000 Units

Item No.  E-DIAEC

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High purity recombinant Diaphorase (Escherichia coli) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

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Application Examples Used widely in diagnostic and analytical applications.
Brand Megazyme
CAS Number 9001-18-7 
Concentration ~ 275 U/mL
EC Number
Enzyme Other Activities
Expression Recombinant from Escherichia coli
Formulation In 3.2 M ammonium sulphate
Molecular Weight 51700
Package Dimensions 0.70 IN x 0.70 IN x 1.60 IN
Package Weight 0.04 LB
pH Optima 9.0-9.4
Physical Form Suspension
Source Escherichia coli
Specificity Catalyses the reaction: Tetrazolium compound + NAD+ →  formazan compound + NADH + H+
Specific Activity ~ 12 U/mg (25°C, pH 9.0)
Storage Conditions

Storage Temperature: 2-8°C

Stability: Minimum 1 year at 4°C. Check vial for details.

Synonyms dihydrolipoyl dehydrogenase; protein-N6-(dihydrolipoyl)lysine:NAD+ oxidoreductase
Temperature Optima (°C) 25
Unit Definition One Unit of diaphorase activity is defined as the amount of enzyme required to oxidise one μmole of NADH per minute with associated reduction of INT in Tris.HCl buffer (32.3 mM), pH 9.0 at 25°C.