Cytidylate kinase (prokaryote)

500 Units

Item No.  E-CMPK

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High purity recombinant Cytidylate kinase (prokaryote) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

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Megazyme_Scheme-E-CMPK.png
Specifications
Application Examples Applications in the measurement of CMP.
Brand Megazyme
Concentration ~ 125 U/mL
EC Number 2.7.4.25
Enzyme Kinase
Expression Recombinant from a Prokaryote
Formulation In 3.2 M ammonium sulphate
Molecular Weight 26910
Package Dimensions 0.70 IN x 0.70 IN x 1.60 IN
Package Weight 0.11 LB
pH Optima 7.5-7.9
Physical Form Suspension
Source Prokaryote
Specificity Catalyses the reaction: ATP + (d)CMP = ADP + (d)CDP. The prokaryotic cytidine monophosphate kinase specifically phosphorylates CMP (or dCMP), using ATP as the preferred phosphoryl donor.
Specific Activity ~ 15 U/mg (25°C, pH 7.6 on CMP)
Storage Conditions

Storage Temperature: 2-8°C

Stability: Minimum 1 year at 4°C. Check vial for details.

Synonyms (d)CMP kinase; ATP:CMP phosphotransferase; UMP-CMP kinase
Temperature Optima (°C) 25
Unit Definition One Unit of cytidylate kinase is defined as the amount of enzyme required to produce one μmole of CDP from CMP and ATP per minute, in the presence of NADH, in TEA buffer (73 mM), pH 7.6 at 25°C.

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