Cellobiohydrolase I (Trichoderma longibrachiatum)
5 Units
Item No. E-CBHI
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High purity Cellobiohydrolase I (Trichoderma longibrachiatum) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
| Specifications | ||
|---|---|---|
| Brand | Megazyme | |
| EC Number | 3.2.1.176 | |
| Enzyme | Cellobiohydrolase | |
| Storage Conditions |
Storage Temperature: 2-8°C |
|
| Application Examples | Applications established in diagnostics and research within the textiles, food and feed, carbohydrate and biofuels industries. | |
| Formulation | In 3.2 M ammonium sulphate | |
| Physical Form | Suspension | |
| CAS Number | 37329-65-0 | |
| CAZy Family | GH7 | |
| Concentration | ~ 0.1 U/mg | |
| Synonyms | cellulose 1,4-beta-cellobiosidase (non-reducing end); 4-beta-D-glucan cellobiohydrolase (non-reducing end) | |
| Source | Trichoderma longibrachiatum | |
| Molecular Weight | 65000 | |
| Expression | Purified from Trichoderma longibrachiatum. | |
| Specificity | Hydrolysis of (1,4)-β-D-glucosidic linkages in cellulose and cellotetraose, releasing cellobiose from the non-reducing ends of the chains. Active on pNP β-lactoside. | |
| Specific Activity | ~ 0.1 U/mg (40°C, pH 4.5 on p-nitrophenyl-β-lactoside) | |
| Unit Definition | One Unit of cellobiohydrolase I activity is defined as the amount of enzyme required to release one µmole of p-nitrophenol (pNP) per minute from p-nitrophenyl-β-lactoside (2.5 mg/mL) in sodium acetate buffer (100 mM), pH 4.5 and 40°C. | |
| Temperature Optima (°C) | 70 | |
| pH Optima | 4.5-4.9 | |
| Package Weight | 0.03 | |