Cellobiohydrolase I (Trichoderma longibrachiatum)

5 Units

Item No.  E-CBHI

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High purity Cellobiohydrolase I (Trichoderma longibrachiatum) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

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Specifications
Application Examples Applications established in diagnostics and research within the textiles, food and feed, carbohydrate and biofuels industries.
Brand Megazyme
CAS Number 37329-65-0
CAZy Family GH7
Concentration ~ 0.1 U/mg
EC Number 3.2.1.176
Enzyme Cellobiohydrolase
Expression Purified from Trichoderma longibrachiatum.
Formulation In 3.2 M ammonium sulphate
Molecular Weight 65000
Package Dimensions 0.80 IN x 0.80 IN x 1.90 IN
Package Weight 0.03 LB
pH Optima 4.5-4.9
Physical Form Suspension
Source Trichoderma longibrachiatum
Specificity Hydrolysis of (1,4)-β-D-glucosidic linkages in cellulose and cellotetraose, releasing cellobiose from the non-reducing ends of the chains. Active on pNP β-lactoside.
Specific Activity ~ 0.1 U/mg (40°C, pH 4.5 on p-nitrophenyl-β-lactoside)
Storage Conditions

Storage Temperature: 2-8°C

Stability: > 4 years at 4°C

Synonyms cellulose 1,4-beta-cellobiosidase (non-reducing end); 4-beta-D-glucan cellobiohydrolase (non-reducing end)
Temperature Optima (°C) 70
Unit Definition One Unit of cellobiohydrolase I activity is defined as the amount of enzyme required to release one µmole of p-nitrophenol (pNP) per minute from p-nitrophenyl-β-lactoside (2.5 mg/mL) in sodium acetate buffer (100 mM), pH 4.5 and 40°C.

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