β-Glucuronidase (Escherichia coli)

500,000 Units

Item No.  E-BGLAEC

This item has shipping restrictions.
$282.00 each
In stock
Out of stock
Expect availability in 2-4 weeks
Qty:
Minimum quantity required: 1
This item must be ordered in multiples of one case. Quantity per case: 1

High purity recombinant β-Glucuronidase (Escherichia coli) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

Do Not Freeze/Thaw 

View Documents

Megazyme_Scheme-E-BGLAEC.png
Specifications
Brand Megazyme
EC Number 3.2.1.31
Enzyme β-Glucuronidase
Storage Conditions

Storage Temperature: 2-8°C

Stability: > 4 years at 4°C

Formulation In solution (Tris.HCl/NaCl/EDTA)
Physical Form Solution
CAS Number 9001-45-0
CAZy Family GH2
Concentration ~ 250 kU/mL
Synonyms β-D-glucuronoside glucuronosohydrolase; GUS
Source Escherichia coli
Molecular Weight 82600
Expression Recombinant from Escherichia coli
Specificity Hydrolysis of non-reducing terminal β-D-glucuronic acid residues from glycoproteins and oligosaccharides of glycoconjugates.
Specific Activity ~ 15,000 U/mg (37°C, pH 6.8 on phenolphthalein-β-D-glucuronide);
~ 50 U/mg (37°C, pH 7.5 on pNP-β-D-glucuronide)
Unit Definition 30000 U/mg protein: One Unit of β-D-glucuronosidase activity is defined as the amount of enzyme required to release one µg of phenolphthalein per hour from phenolphthalein-β-D-glucuronide (0.5 mM) in sodium phosphate buffer (100 mM) at pH 6.8 and 37°C.
110 U/mg protein: One Unit of β-D-glucuronosidase activity is defined as the amount of enzyme required to release one µmole of p-nitrophenol per minute from pNP-β-D-glucuronide (1 mM) in Tris.HCl buffer (100 mM) pH 7.5 and 37°C., monitored at 410 nm.
Temperature Optima (°C) 37
pH Optima 6.5-6.9
Package Dimensions 0.70 in x 0.70 in x 1.60 in
Package Weight 0.03 lb

Support