β-Galactosidase (Aspergillus niger)
8,000 Units
Item No. E-BGLAN
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High purity β-Galactosidase (Aspergillus niger) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
| Specifications | ||
|---|---|---|
| Brand | Megazyme | |
| EC Number | 3.2.1.23 | |
| Enzyme | β-Galactosidase | |
| Storage Conditions |
Storage Temperature: 2-8°C Stability: > 4 years at 4°C |
|
| Application Examples | Applications established in diagnostics and research within the food and feed, carbohydrate and biofuels industries. | |
| Formulation | In 3.2 M ammonium sulphate | |
| Physical Form | Suspension | |
| CAS Number | 9013-11-2 | |
| CAZy Family | GH35 | |
| Concentration | ~ 4000 U/mL | |
| Synonyms | beta-galactosidase; beta-D-galactoside galactohydrolase | |
| Source | Aspergillus niger | |
| Molecular Weight | 125000 | |
| Expression | Purified from Aspergillus niger | |
| Specificity | Hydrolysis of terminal non-reducing β-D-galactose residues in β-D-galactosides. | |
| Specific Activity | ~ 170 U/mg (40°C, pH 4.5 on p-nitrophenyl β-D-galactoside) | |
| Unit Definition | One Unit of β-galactosidase activity is defined as the amount of enzyme required to release one µmole of p-nitrophenol (pNP) per minute from p-nitrophenyl-β-D-galactoside (10 mM) in sodium acetate buffer (100 mM), pH 4.5 at 40°C. | |
| Temperature Optima (°C) | 60 | |
| pH Optima | 5.0-5.4 | |
| Package Weight | 0.03 | |