Asparaginase (Escherichia coli)

400 Units at 25°C

Item No.  E-ASNEC

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High purity recombinant Asparaginase (Escherichia coli) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

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Specifications
Application Examples Applications established for the measurement of asparagine in the food, fermentation, wine, beverage, medical and pharmacological industries.
Brand Megazyme
CAS Number 9015-68-3 
Concentration ~ 350 U/mL
EC Number 3.5.1.1
Enzyme Other Activities
Expression Recombinant from Escherichia coli
Formulation In 2.5 M lithium sulphate
Molecular Weight 37900
Package Dimensions 0.70 IN x 0.70 IN x 1.60 IN
Package Weight 0.04 LB
pH Optima 8.0-8.4
Physical Form Suspension
Source Escherichia coli
Specificity Catalyses the reaction: L-asparagine + H2O = L-aspartate + NH3
Specific Activity ~ 15 U/mg (25°C, pH 8.0 on L-asparagine)
~ 30 U/mg (37°C, pH 8.0 on L-asparagine)
Storage Conditions

Storage Temperature: 2-8°C

Stability: > 4 years at 4°C

Synonyms asparaginase; L-asparagine amidohydrolase
Temperature Optima (°C) 37
Unit Definition One Unit of asparaginase activity is defined as the amount of enzyme required to produce one μmole of L-aspartate from L-asparagine (7.3 mM) per minute in the presence of NADPH in Tris.HCL buffer (45 mM), pH 8.0.

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