α-L-Arabinofuranosidase (Ustilago maydis)

400 Units on WAX at 40°C

Item No.  E-ABFUM

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High purity α-L-Arabinofuranosidase (Ustilago maydis) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

Highly active on wheat arabonoxylan.

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Specifications
Application Examples For use in plant cell wall carbohydrate and biofuels research.
Brand Megazyme
CAS Number 9067-74-7
CAZy Family GH62
Concentration ~ 200 U/mL
EC Number 3.2.1.55
Enzyme α-Arabinofuranosidase
Expression Recombinant from Ustilago maydis
Formulation In 3.2 M ammonium sulphate
Molecular Weight 35000
Package Dimensions 0.70 IN x 0.70 IN x 1.60 IN
Package Weight 0.03 LB
pH Optima 5.0-5.4
Physical Form Suspension
Source Ustilago maydis
Specificity Hydrolysis of terminal, non-reducing α-L-arabinofuranose from singly substituted xylose residues in arabinoxylan (α-1,2 > α-1,3).  Does not hydrolyse α-L-arabinofuranose from doubly substituted xylose residues in arabinoxylan.
Specific Activity ~ 9 U/mg protein (40°C, pH 5.0 on wheat arabinoxylan)
Storage Conditions

Storage Temperature: 2-8°C

Stability: Minimum 1 year at 4°C. Check vial for details.

Synonyms non-reducing end alpha-L-arabinofuranosidase; alpha-L-arabinofuranoside non-reducing end alpha-L-arabinofuranosidase
Temperature Optima (°C) 40
Unit Definition One Unit of α-L-arabinfuranosidase activity is defined as the amount of enzyme required to release one μmole of arabinose per minute from wheat arabinoxylan (10 mg/mL) in sodium acetate buffer (100 mM), pH 5.0 at 40°C.

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