α-L-Arabinofuranosidase (Aspergillus niger)
480 Units on pNP-α-Arabf at 40oC
Item No. E-AFASE
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High purity α-L-Arabinofuranosidase (Aspergillus niger) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
| Specifications | ||
|---|---|---|
| Brand | Megazyme | |
| EC Number | 3.2.1.55 | |
| Enzyme | α-Arabinofuranosidase | |
| Storage Conditions |
Storage Temperature: 2-8°C |
|
| Application Examples | Applications in carbohydrate and biofuels research. | |
| Formulation | In 3.2 M ammonium sulphate | |
| Physical Form | Suspension | |
| CAS Number | 9067-74-7 | |
| CAZy Family | GH51 | |
| Concentration | ~ 300 | |
| Synonyms | non-reducing end alpha-L-arabinofuranosidase; alpha-L-arabinofuranoside non-reducing end alpha-L-arabinofuranosidase | |
| Source | Aspergillus niger | |
| Molecular Weight | 62000 | |
| Expression | From Aspergillus niger | |
| Specificity | Hydrolysis of α-1,2- and α-1,3-linked L-arabinofuranose residues from arabinoxylans and branched arabinans. Hydrolyses α-1,5-linked arabino-oligosaccharides at a much lower rate. | |
| Specific Activity |
~ 32 U/mg (40°C, pH 5.5 on p-nitrophenyl-α-L-arabinofuranoside); ~ 12.6 U/mg (on sugar-beet arabinan); ~ 1.2 U/mg (on wheat arabinoxylan) |
|
| Unit Definition | One Unit of α-L-arabinofuranosidase activity is defined as the amount of enzyme required to release one µmole of p-nitrophenol (pNP) per minute from p-nitrophenyl-α-L-arabinofuranoside (5 mM) in sodium acetate buffer (100 mM), pH 4.0 at 40°C. | |
| Temperature Optima (°C) | 40 | |
| pH Optima | 4.0-4.4 | |
| Package Weight | 0.03 lb | |
Documents
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