α-L-Arabinofuranosidase (Aspergillus niger)

480 Units on pNP-α-Arabf at 40oC

Item No.  E-AFASE

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High purity α-L-Arabinofuranosidase (Aspergillus niger) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

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Specifications
Brand Megazyme
EC Number 3.2.1.55
Enzyme α-Arabinofuranosidase
Storage Conditions

Storage Temperature: 2-8°C

Stability: > 4 years at 4°C

Application Examples Applications in carbohydrate and biofuels research.
Formulation In 3.2 M ammonium sulphate
Physical Form Suspension
CAS Number 9067-74-7
CAZy Family GH51
Concentration ~ 300 U/mL
Synonyms non-reducing end alpha-L-arabinofuranosidase; alpha-L-arabinofuranoside non-reducing end alpha-L-arabinofuranosidase
Source Aspergillus niger
Molecular Weight 62000
Expression From Aspergillus niger
Specificity Hydrolysis of α-1,2- and α-1,3-linked L-arabinofuranose residues from arabinoxylans and branched arabinans. Hydrolyses α-1,5-linked arabino-oligosaccharides at a much lower rate.
Specific Activity ~ 32 U/mg (40°C, pH 5.5 on p-nitrophenyl-α-L-arabinofuranoside);
~ 12.6 U/mg (on sugar-beet arabinan);
~ 1.2 U/mg (on wheat arabinoxylan)
Unit Definition One Unit of α-L-arabinofuranosidase activity is defined as the amount of enzyme required to release one µmole of p-nitrophenol (pNP) per minute from p-nitrophenyl-α-L-arabinofuranoside (5 mM) in sodium acetate buffer (100 mM), pH 4.0 at 40°C.
Temperature Optima (°C) 40
pH Optima 4.0-4.4
Package Dimensions 0.70 in x 0.70 in x 1.60 in
Package Weight 0.03 lb

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