α-Glucosidase (Bacillus stearothermophilus) (Recombinant)
3,000 Units at 40°C
Item No. E-TSAGS
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High purity recombinant α-Glucosidase (Bacillus stearothermophilus) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
| Specifications | ||
|---|---|---|
| Brand | Megazyme | |
| EC Number | 3.2.1.20 | |
| Enzyme | α-Glucosidase | |
| Storage Conditions |
Storage Temperature: 2-8°C Stability: > 4 years at 4°C |
|
| Application Examples | Applications in carbohydrate and biofuels research and diagnostic and analytical procedures. | |
| Formulation | In 3.2 M ammonium sulphate | |
| Physical Form | Suspension | |
| CAS Number | 9001-42-7 | |
| CAZy Family | GH13 | |
| Concentration | ~ 1500 U/mL | |
| Synonyms | alpha-glucosidase; alpha-D-glucoside glucohydrolase | |
| Source | Bacillus stearothermophilus | |
| Molecular Weight | 66000 | |
| Expression | Recombinant from Bacillus stearothermophilus | |
| Specificity | Hydrolysis of terminal, non-reducing α-1,4-linked D-glucose residues with release of D-glucose. | |
| Specific Activity | ~ 60 U/mg (40°C, pH 6.5 on p-nitrophenyl-α-D-glucopyranoside). | |
| Unit Definition | One Unit of α-D-glucosidase activity is defined as the amount of enzyme required to release one µmole of p-nitrophenol per minute from 4-nitrophenyl α-D-glucopyranoside (5 mM), in sodium phosphate buffer (100 mM), pH 6.5 at 40°C. | |
| Temperature Optima (°C) | 60 | |
| pH Optima | 6.5-6.9 | |
| Package Weight | 0.03 | |