α-(1-2,3,4,6)-L-Fucosidase (Homo sapiens)

10 Units at 25°C (~ 34 Units at 37°C)

Item No.  E-FUCHS

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High purity α-(1-2,3,4,6)-L-Fucosidase (Homo sapiens) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

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Specifications
Application Examples For use in glycobiology research.
Brand Megazyme
CAS Number 9037-65-4
CAZy Family GH29
Concentration ~ 50 U/mL
EC Number 3.2.1.51
Enzyme α-Fucosidase
Expression Recombinant from Homo sapiens
Formulation In 3.2 M ammonium sulphate
Molecular Weight 52000
Package Dimensions 0.70 IN x 0.70 IN x 1.60 IN
Package Weight 0.00 LB
pH Optima 4.0-4.4
Physical Form Suspension
Source Homo sapiens
Specificity Broad specificity; hydrolysis of terminal non-reducing α-(1-2,3,4,6)-linked L-fucose residues from glycoproteins and oligosaccharides.
Specific Activity ~ 5 U/mg protein (on pNP-α-L-fucopyranoside) at pH 4.0 and 25°C;
~ 16 U/mg at 37°C;
~ 48 U/mg at 50°C.
Storage Conditions

Storage Temperature: 2-8°C

Stability: Minimum 1 year at 4°C. Check vial for details.

Synonyms alpha-L-fucosidase; alpha-L-fucoside fucohydrolase
Temperature Optima (°C) 50
Unit Definition One Unit of α-L-fucosidase activity is defined as the amount of enzyme required to release one µmole of p-nitrophenol (pNP) per minute from p-nitrophenyl-α-L-fucopyranoside (1 mM) in sodium acetate buffer (100 mM) at pH 4.0 at the temperatures indicated.

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