6-Phosphogluconate dehydrogenase (Escherichia coli)

150 Units

Item No.  E-PGDHEC

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High purity recombinant 6-Phosphogluconate dehydrogenase (Escherichia coli) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

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Specifications
Application Examples Applications in diagnostics and analytical methods in the food and feeds, fermentation beverages and wine industries.
Brand Megazyme
CAS Number 9073-95-4 
Concentration ~ 50 U/mL
EC Number 1.1.1.44
Enzyme Dehydrogenase
Expression Recombinant from Escherichia coli
Formulation In 3.2 M ammonium sulphate
Molecular Weight 52546
Package Dimensions 0.70 IN x 0.70 IN x 1.60 IN
Package Weight 0.11 LB
pH Optima 7.5-7.9
Physical Form Suspension
Source Escherichia coli
Specificity Catalyses the reaction: 6-phospho-D-gluconate + NADP+ = D-ribulose 5-phosphate + CO2 + NADPH + H+
Specific Activity ~ 9 U/mg (25°C, pH 7.6 on D-gluconic acid)
Storage Conditions

Storage Temperature: 2-8°C

Stability: Minimum 1 year at 4°C. Check vial for details.

Synonyms phosphogluconate dehydrogenase (NADP+-dependent, decarboxylating); 6-phospho-D-gluconate: NADP+ 2-oxidoreductase (decarboxylating)
Temperature Optima (°C) 25
Unit Definition One unit of 6-phosphogluconate dehydrogenase activity is the amount of enzyme required to convert one μmole of 6-phospho gluconic acid to D-ribulose 5-phosphate per min in TEA buffer (86 mM), pH 7.6 at 25°C.

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