Veratox® for Tetracycline

Item No.  DR016

  • Accurate and easy-to-use
  • Cost effective microwell format
  • Broad detection of tetracycline antibiotic class
This item has shipping restrictions.
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Expect availability in 2-4 weeks
Minimum quantity required: 1
This item must be ordered in multiples of one case. Quantity per case: 1
Veratox® for Tetracycline provides a rapid, high recovery, cost-effective and competitive enzyme immunoassay for the quantitative analysis of Tetracycline in muscle.
Analyte Tetracycline
Brand Veratox®
Package Dimensions 5.90 IN x 3.30 IN x 6.70 IN
Package Weight 0.80 LB
Platform ELISA
Quantity per Package 36 tests
Result Type Quantitative
Testing Time Minutes
Time to Result Minutes

  1. Tetracycline-coated Plate 1 x 96-well plate (8 wells x 12 strips)
  2. Tetracycline Standard:
    1. 0 ng/mL (white cap tube) 1.2 mL
    2. 0.05 ng/mL (yellow cap tube) 1.2 mL
    3. 0.15 ng/mL (orange cap tube) 1.2 mL
    4. 0.4 ng/mL (pink cap tube) 1.2 mL
    5. 0.8 ng/mL (purple cap tube) 1.2 mL
    6. 1.6 ng/mL (blue cap tube) 1.2 mL
    7. 100 ng/mL (spiking, red cap tube) 1.2 mL
  3. Tetracycline Antibody #1 12mL
  4. 100X HRP-Conjugated Antibody #2 300 µL
  5. Antibody #2 Diluent 20 mL
  6. 20X Wash Solution 28 mL
  7. Stop Buffer 14 mL
  8. TMB Substrate 12 mL
  9. 5X OXYTET Extraction Buffer 2 x 25 mL
  10. 10X TET Sample Diluent 28 mL
  11. TET Balance Buffer Concentrate 5 mL
  1. Microtiter plate reader with a 450 nm filter (Neogen® item 9303)
  2. 20–200 µL pipettes (Neogen item 9276)
  3. Multichannel pipette: 50–300 µL (optional) (Neogen item 9385)
  4. 1000 µL pipettor (Neogen 9337)
  5. Vortex Mixer (Neogen 9494)
  6. Centrifuge 4,000 x g
  7. Timer (Neogen item 9426)
  8. Wash bottle (Neogen item 9400)
  9. Paper towels or equivalent absorbent material
  10. Veratox software (Neogen item 9305)
  11. Lab station (Neogen item 9481)
  12. Distilled or deionized water
  13. Blender or food processor to homogenize sample


The method is based on a competitive colorimetric ELISA assay. The drug of interest has been coated in the plate wells. During the analysis, sample is added along with the primary antibody specific for the target drug. If the target is present in the sample, it will compete for the antibody, thereby preventing the antibody from binding to the drug attached to the well. The secondary antibody, tagged with a peroxidase enzyme, targets the primary antibody that is complexed to the drug coated on the plate wells. The resulting color intensity, after addition of substrate, has an inverse relationship with the target concentration in the sample.


Our customers’ success is our shared success. Our experts are ready to train you and your team on our solutions, so you can rest easy knowing procedures are performed properly and yield accurate results. In addition, we provide certificates upon completion of training to provide you with the documentation needed for audit traceability.



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