Fructanase Mixture (Ultrapure, recombinant, liquid)
10 ml / 20,000 Units
Item No. E-FRLQPU
High purity Fructanase Mixture (liquid) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
For Fructan Determination.
Fructanase, Amyloglucosidase (E-AMGDF) and Isoamylase (E-ISAMY) are used in the enzyme hydrolysis step of the AOAC method 2001.11 for the determination of polydextrose (a low molar mass dietary fiber) in foods.
Components: exo-Inulinase (2,000 U/mL on kestose, at 40°C) and endo-Inulinase (100 U/mL on inulin at 40°C).
Note: This product is a mixture of two ultrapure, recombinant enzymes. Suitable for the measurement of fructan (AOAC Method 997.08) and in the measurement of Polydextrose (AOAC Method 2000.11).
| Specifications | ||
|---|---|---|
| Brand | Megazyme | |
| EC Number |
endo-Inulinase: 3.2.1.7, exo-Inulinase: 3.2.1.80 |
|
| Enzyme | Fructanase | |
| Storage Conditions |
Storage Temperature: Below -10°C Stability: Minimum 1 year at < -10°C. Check vial for details |
|
| Application Examples | Applications in carbohydrate research and in the food industry. | |
| Formulation | In 50% (v/v) glycerol plus 0.02% sodium azide | |
| Physical Form | Solution | |
| CAS Number |
9001-57-4, 9025-67-6, 37288-56-5 |
|
| CAZy Family | GH32 | |
| Concentration |
endo-inulinase: Supplied at ~ 1,000 U exo-inulinase: Supplied at ~ 10,000 U |
|
| Synonyms |
endo-inulinase: 1-beta-D-fructan fructanohydrolase exo-inulinase: fructan β-fructosidase |
|
| Source | Aspergillus niger | |
| Expression | Recombinant from Aspergillus niger | |
| Specificity |
endo-Inulinase: endo-acting hydrolysis of (2,1)-β-D-fructosidic linkages in inulin. exo-Inulinase: Hydrolysis of terminal, non-reducing β-D-fructofuranoside residues in β-D-fructofuranosides. |
|
| Unit Definition |
endo-Inulinase: One Unit of endo-inulinase activity is defined as the amount of enzyme required to release one μmole of β-D-fructose reducing-sugar equivalents per minute from inulin (20 mg/mL) in sodium acetate buffer (100 mM), pH 4.5. exo-Inulinase: One Unit of exo-inulinase activity is defined as the amount of enzyme required to release one μmole of β-D-fructose reducing-sugar equivalents per minute from kestose (5 mg/mL) in sodium acetate buffer (100 mM), at pH 4.5 at 40°C. |
|
| Temperature Optima (°C) | 60 | |
| pH Optima | 4.5-4.9 | |
| Package Weight | 0.06 lb | |
Documents
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